The Inhibition of Cyanobacteria Spirulina Extract on S. Mutans Biofilm as an Ingredient in Mouthwash

Authors

  • Henu Sumekar Departement of Conservative Dentistry, Faculty of Dentistry, Universitas Hang Tuah
  • Linda Rochyani Departement of Conservative Dentistry, Faculty of Dentistry, Universitas Hang Tuah
  • Istien Wardani Departement of Pediatric Dentistry, Faculty of Dentistry, Universitas Hang Tuah

DOI:

https://doi.org/10.30649/denta.v18i2.8

Keywords:

Cyanobacteria spirulina, Chlorhexidine, Mouth Wash, S. Mutans Biofilm

Abstract

Background: The main cause of dental caries is Streptococcus mutans (S. mutans), to suppress the growth of cariogenic bacteria can be done by using mouthwash containing antibacterial ingredients such as chlorhexidine. Cyanobacteria spirulina extract contains compounds that have secondary metabolites which act as antibacterial and antioxidant including tannins, flavonoids, saponins and terpenoids. Objective: This study aimed to find out he inhibition of Cyanobacteria spirulina against S. mutans biofilm. Materials and Methods: The research sample consisted of 6 groups. Group K(-) was the S. mutans biofilm with aquadest, K(+) was Chlorhexidine 0.2% and four treatment groups (P1, P2, P3, P4), which were Cyanobacteria spirulina extract with a concentration of 60 , 70, 80 and 90 mg/ml. The sample was applied to a well microtiter plate, then incubated overnight at 37°C for 1 x 24 hours, then painted with 0.1% crystal violet. After that, it was rinsed with sterile distilled water, then given 0.2 mL of Tween 80 concentration of 2% and measured Optical Density with an ELISA Reader with a wavelength of 570 nm, then biofilm inhibition percentage as calculated. Results: The mean value of the percentage of S. mutans biofilm inhibition in group K(+): -131,694(SD 10,758) ; P1: -12,234(SD 2,402) ; P2: 11,076(SD 6,387) ; P3: 19.7020(SD 11,670) ; P4: 40,214(12,057) there were significant differences between P2 and P4 also between P3 and P4. Conclusion: Cyanobacteria spirulina at concentrations of 70, 80 and 90 mg/ml had S. mutans biofilm inhibition, while Chlorhexidine 0.2% and Cyanobacteria spirulina 60 mg/ml did not.

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References

Tarigan R. 2013. Perawatan Pulpa Gigi Endodontik. Ed. 3 EGC. Jakarta. h. 28-39.

Owu, N. M. dan Jayanti, M. 2020. Uji Efektivitas Penghambatan Dari Ekstrak Daun Sirih (Piper Betle L.) Terhadap Bakteri Streptococcus Mutans. Jurnal Biomedik : Jbm, 12(3), pp. 145–152. Doi: 10.35790/Jbm.12.3.2020.29185.

Purbowati, R. 2018. Hubungan biofim dengan infeksi : Implikasi pada kesehatan masyarakat dan strategi mengontrolnya. Jurnal Ilmiah Kedokteran, 5(1): pp. 1-14.

Peng X, Ekanayaka SA, McClellan SA, Barrett RP, Vistien K, Hazlett LD, 2017. Characterization of Three Ocular Clinical isolates of P. aeruginosa: Viability, Biofilm Formation, Adherence, Infectivity, and Effects of Glycyrrhizin. MDPI Journal Patogens 6(4): pp. 1-17.

Vasudevan R, 2017. Dental Plaques: Microbial Community of the Oral Cavity. Journal of Microbiology & Experimentation 4(1): pp. 1-9.

Oilo M, Bakken V, 2015. Biofilm and Dental Materials. MDPI Materials 8: pp. 2887-2900.

Kurniawati, A. 2018. ‘Pengaruh Kumur Ekstrak Daun Ungu Terhadap Jumlah Bakteri Dalam Saliva (The Effect Of Gargling Purple Leaves Extract On The Number Of Bacteria In Saliva)’, Stomatognatic(J.K.G), 15(2), pp. 43-46.

Sinaredi, B. R., Pradopo, S., Wibowo, T. B. 2014. Daya Antibakteri Obat Kumur Chlorhexidine, Povidone Iodine, Fluoride Suplementasi Zinc Terhadap, Streptococcus Mutans Dan Porphyromonas Gingivalis (Antibacterial Effect Of Mouth Washes Containing Chlorhexidine, Povidone Iodine, Fluoride Plus Zinc On Strep). Dental Journal (Majalah Kedokteran Gigi), 47(4), pp. 211. Doi: 10.20473/J.Djmkg.V47.I4.P211-214.

Kim, S.K. 2020. Encyclopedia of Marine Biotechnology: Five Volume Set, 1st ed. John Wiley & Sons Ltd. Published, pp 2161-2162.

Diah Astika Winahyu, Agustina Retnaningsih, S. K. 2020. Uji Aktivitas Antibakteri Ekstrak Spirulina Platensis Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Dan Propionibacterium Acne Dengan, Jurnal Analisi Farmasi, 5(2), pp. 118-126.

El-Sheekh, M.M., Daboor, S.M., Swelim, M.A., Mohamed, S. 2014. Production and Characterization of Antimicrobial Active Substance From Spirulina platensis. Iran. J. Microbiol. 6(2): pp.112-119.

Gheda, S.F., And Ismail, G.A. 2020. Natural Products from some Soil Cyanobacterial Extracts With Potent Antimicrobial, Antioxidant and Cytotoxic Activities. An Acad Bras Cienc. 92(2): pp. 1-18.

Faisal alsenani, Karnaker R. Tupally, Elvis T. Chua, Eladi Eltanaly, Hamed Alsufyani, Harendra S. Parekh, and Peer M. Schen (2020) ‘Evaluation Of microalgae and cyanobacteria as potential source of antimicrobial compounds’, Saudi Pharmaceutical journal, Vol 28, pp. 1834-18941.

Mostafa M. El-Sheekh, Said M. Daboor, Mahmoud A. Swelim, Soha Mohamed. 2014. Production and characterization of antimicrobial active substance from Spirulina platensis, Journal

Suhendar, U. dan Fathurrahman, M. 2019. Aktivitas Antibakteri Ekstrak Metanol Bunga Cengkeh (Syzygium Aromaticum) Terhadap Bakteri Streptococcus Mutans. FITOFARMAKA: Jurnal Ilmiah Farmasi, 9(1), pp. 26–34. Doi: 10.33751/Jf. V9i1.1257.

Hasanuddin, A. Rp., Salnus, S. 2020. Uji Bioaktivitas Minyak Cengkeh (Syzygium Aromaticum) Terhadap Pertumbuhan Bakteri Streptococcus Mutans Penyebab Karies Gigi, Jurnal Biologi Makassar, 5(2), pp. 241–250.

Winahyu, D.A., Retnaningsih, A., Koriah, S. 2020. Uji Aktivitas Antibakteri Ekstrak Spirulina platensis Terhadap Pertumbuhan Bakteri Staphylococcus aureus Dan Proprionibacterium acne Dengan Metode Difusi Agar . Jurnal Analisis Farmasi. 5(2): pp. 118-126.

Davoodbasha M., Edachery B., Nooruddin T., Lee S.Y., Kim J.W. 2018. An evidence of C16 fatty acid methyl esters extracted from microalga for effective antimicrobial and antioxidant property. Int. Journal Microb. Pathog.;115:233–238.

Mudaliana, S. (2021). Antimicrobial activity of Centella asiatica and Gigantochloa apus.Journal of Basic and Clinical Physiology and Pharmacology, 32(4), 755–759. https://doi.org/10.1515/jbcp p-2020-0396

Makarewicz, M., Drożdż, I., T arko, T ., & Duda-Chodak, A. (2021). The Interactions between Polyphenols and Microorganisms, Especially Gut Microbiota. Antioxidants, 10(2), 188. https://doi.org/10.3390/antio x10020188

Sudarmi, K., Darmayasa, I. B. G., & Muksin, I. K. (2017). Uji fitokima dan daya hambat ekstrak daun juwet (Syzygium cumini) terhadap pertumbuhan Escherichia coli danStaphylococcus aureus A TCC. SIMBIOSIS, 5(2), 47–51. https://doi.org/10.24843/JSI MBIOSIS.2017.v05.i02.p03

Sun, B., Wu, L., Wu, Y., Zhang, C., Qin, L., Hayashi, M., Kudo, M., Gao, M., & Liu, T . (2020). Therapeutic Potential of Centella asiatica and Its Triterpenes: A Review. Frontiers in Pharmacology, 11, 568032. https://doi.org/10.3389/fphar .2020.568032

Madhumathi V., Deepa P., Jeyachandran S., Manoharan C., Vijayakumar S. 2011. Antimicrobial Activity of Cyanobacteria Isolated from Freshwater Lake. Int. Journal Microbiol. Res.; 2:213–216

Wu Q., Liu L., Miron A., Klímova B., Wan D., Kuca K. 2016. The antioxidant, immunomodulatory, and anti-inflammatory activities of Spirulina: an overview. Journal Arch. Toxicol. ; 90(8):1817–1840.

Al-Ghanayem A. A. 2017. Antimicrobial activity of Spirulina platensis extracts against certain pathogenic bacteria and fungi. Adv. Biores., 8(6) November, pp. 96-101.

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Published

2024-08-31

How to Cite

Henu Sumekar, Linda Rochyani, & Istien Wardani. (2024). The Inhibition of Cyanobacteria Spirulina Extract on S. Mutans Biofilm as an Ingredient in Mouthwash. DENTA JURNAL KEDOKTERAN GIGI, 18(2), 107–113. https://doi.org/10.30649/denta.v18i2.8

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Vol. 18 No. 2 (2024): Agustus

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