The Antibacterial Effect of Anchovy (Stolephorus Insularis) Extract Against Enterococcus Faecalis
Keywords:
Enterococcus faecalis, root canal sterilization agent, anchovy (Stolephorus insularis)Abstract
Backgrounds: Enterococcus faecalis is a facultative anaerobic gram-positive bacteria that known to have a role in the infection of the root canal, causing the failure of root canal treatment. The extract from anchovy (Stolephorus insularis) contains fluor has antibacterial properties that can inhibit the growth of gram-positive bacteria and has the potential to be developed as a root canal sterilization agent. Purpose : To determine the antibacterial effect of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. Methods : This study was an true experimental study with post test only control group design and were tested by diffusion methods, consisted of 5 groups: 2 control groups DMSO 1% as negative control and ChKM as positive control, 3 treatment groups of anchovy (Stolephorus insularis) extract with different concentrations of 18%, 24% and 30% which each group consisted of 5 samples. Antivbacterial effect was examined by measuring the clear zone around the filter paper. Data were analyzed by ANOVA test followed by LSD test. Results : Results of this study proved the presence of antibacterial effects of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. In this study, the higher concentrations used, the greater the antibacterial effect. Average inhibition zone of each groups: DMSO 1% (6,03 mm), ChKM (14,18 mm), 18%(6,83 mm), 24%(7,03 mm) and 30% (7,16 mm). Conclusion : Anchovy (Stolephorus insularis) extract has antibacterial effect to the Enterococcus faecalis.
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Kurniawati SW, 2008. Aktivitas Antibakteri dari Ekstrak Etanol Daun Asam Jawa (Tamarindus Indica Lin) Terhadap Kultur Aktif Staphylococcus aureus dan Escherichia colli. Skripsi Universitas Negeri Syarif Hidayatullah Jakarta, h: 50
Worotitjan I, Mintjelungan CN, Gunawan P, 2013. Pengalaman Karies Gigi serta Pola Makan dan Minum pada Anak Sekolah Dasar di Desa Kiawa Kecamatan Kawangkoan Utara. Jurnal e-GIGI (eG) 1 (1): 60
Wala HC, Wicaksono DA, Tambunan E, 2014. Gambaran Status Karies Gigi Anak Usia 11-12 Tahun pada Keluarga Pemegang Jamkesmas di Kelurahan Tumatangtang I Kecamatan Tomohon Selatan. Kandidat Skripsi, Fakultas Kedokteran Gigi Universitas Sam Ratulangi Manado, h: 2
Tarigan, R, 1995. Kesehatan Gigi dan Mulut. Cetakan Ke Empat, Penerbit Buku Kedokteran EGC, Jakarta.
Mulyawati E, 2011. Peran Bahan Disinfeksi Pada Perawatan Saluran Akar. Maj Ked Desember 18(2): 205-209
Yustina AR, Suardita K, W Dian A, 2012. Peningkatan jumlah osteoklas pada keradangan periapikal akibat induksi lipopolisakarida Porphyromonas Gingivalis (suatu penelitian laboratoris menggunakan tikus) JBP Vol. 14(3): 140-144
Walton RE, Torabinejad M, 2008. Prinsip & praktek ilmu endodonsia. Ed.3. Penerbit buku kedokteran EGC, h: 36, 205 230, 324-326
Hojo K, Nagaoka S, Oshima T, Maeda N, 2009. Bacterial Interactios in Dental Biofilm Development. J Dent Res 88(11): 982-990
Stuart CH, Schwartz SA, Beeson TJ, Owatz CB, 2006. Enterococcus Faecalis: Its role in root canal treatment failure and current concpetsbin retreatment. Journal of endodontics, 32(2): 93-98
Preethee T, Kndaswamy D, Hannah R, 2012. Molecular identification of an Enterococcus faecalis endocarditis antigen efaA in root canals of therapy-resistant endodontic infections. J Conserv Dent. Vol 15(4) : 319-322
Wintarsih, O., Partosoedarmo, M., Santoso, P., 2009, Kebocoran Apikal pada Irigasi dengan EDTA Lebih Kecil Dibandingkan yang Tanpa EDTA (A Comparative Study of Apical Leakage on Irrigation Using and Without EDTA), Jurnal PDGI., 58 (2) : 14-19
Osswald R, 2005. The Problem of Endodontis and Managing it Trough Conservative Dentistry, p 134-144
Fa’izah A.2016. Konsentrasi Efektif Ekstrak Ikan Teri Jengki (Stolephorus insularis) Sebagai Antimikroba Terhadap Bakteri Enterococcus faecalis. Skripsi, Fakultas Kedokteran Gigi Universitas Hang Tuah Surabaya, h: 30-41
Daradjatun AN, 2014. Pemanfaatan Tepung Kepala Ikan Teri Jengki (Stolephorus insularis) sebagai Bahan Subtitusi Tepung Ikan dalam Pakan Buatan Ikan Lele Masamo (Clarias sp.). Skripsi, Fakultas Pertanian Universitas Lampung, h: 7
Gunawan HA, 2006. Pengaruh Tingkat pH Larutan Teri terhadap Perubahan Dimensi dan Kelarutan Kristal Apatit. Jurnal Anatomi Indonesia, 01(1): 25-29
Mardjudo A, Rahman ARA, 2014. Usaha Perikanan Ikan Teri (Stolephorus sp.) dengan Alat Tangkap Bagan Tancap di Desa Bukit Aru Indah di Kecamatan Sebatik Timur Kabupaten Nunukan Provinsi Kalimantan Utara. Jurnal Ilmiah AgrIBA (2): 198
Hermawan A, 2007. Pengaruh Ekstrak Daun Sirih (Piper betle L) Terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli Dengan Metode Difusi disk. Fakultas Kedokteran Gigi Universitas Airlangga. Jakarta: Salemba Medika, h: 3-4
Zabrina S, 2012. Pengaruh Aplikasi Substrat Ikan Teri Jengki (Stolephorus insularis) terhadap Tingkat Retensi Fluor pada Email Gigi Tikus Sprague Dawley (in Vivo). Skripsi, Fakultas Kedokteran Gigi Universitas Indonesia, h: 1-2, 22
Uttley AHC, George RC, Naidoo J, Woodford N, Johnson AP, Collins CH et al, 2009. High-level Vancomycin-resistant Enterococci Causing Hospital Infections. Epidem. Inf, Great Britain, 103:173-181
Bauman RW, 2004. Microbiology International Edition. San Fransisco: Pearson Education Inc and Person Benjamin Cummings, h: 297-299
Handayani D, Maipa D, Marlina, Meilan, 2009. Skrining Aktivitas Antibakteri Beberapa Biota Laut dari Perairan Pantai Painan, Sumatera Barat. Jurnal, Fakultas Farmasi Universitas Andalas, h: 5
Wadhwani T, K Desai, D Patel, D Lawani, P Bahaley, P Joshi, V Kothari, 2008. Effect of various solvents on bacterial growth in context of determining MIC of antimicrobials. The Internet Journal of Microbiology. 7(1)
Karina VN, 2012. Perbedaan Daya Antibakteri Bahan Perekat Braket Ortodonsi antara Semen Ionomer Kaca dengan Resin Komposit Berfluor terhadap Lactobacillus acidophilus. Skripsi, Fakultas Kedokteran Gigi Universitas Jember, h: 17-18
Handini AD, 2013. Perbedaan Topikal Aplikasi Bahan Casein Phosphopeptide-Amorphous Calcium Phospate (CPP-ACP) dan Bahan Sodium Fluoride terhadap Jumlah Koloni Streptococcus mutans pada Saliva Anak Usia 6-12 Tahun. Skripsi, Fakultas Kedokteran Gigi Universitas Hasanuddin, h: 13-16
Oktanovia SY, 2013. Ikan Teri Dapat Meningkatkan Ketahanan Email Gigi. Makalah Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang, h: 9
Cooper GM, Hausman RE, 2004. The Cell: A Molecular Aproach 3rd ed. Washinton D.C: ASM Press. h: 518 519
Poeloengan M, 2012. Penguian Yoghurt Probiotik pada Pertumbuhan Bakteri. Balai Besar Penelitian Veteriner. h: 303 307
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