Pengaruh Pemberian Ekstrak Nannochloropsis oculata Terhadap Penurunan Kadar TNF-α pada Tikus yang Diinduksi Bakteri Actinobacillus actinomycetemcomitans

Authors

  • Peggie Octavia Halim Sarjana Kedokteran Gigi Universitas Hang Tuah
  • Syamsulina Revianti Biologi Oral Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Yoifah Rizka Wedarti Periodonsia Fakultas Kedokteran Gigi Universitas Hang Tuah

Keywords:

Nannochloropsis oculata, Actinobacillus actinomycetemcommitans, alveolar bone resorption

Abstract

Background: Actinobacillus actinomycetemcomitans is a major periodontopathic bacterium with multiple virulence factors, including LPS. LPS is a potent stimulator cytokines proinflammatory such as TNF-α. Nannochloropsis oculata is a green micro algae that has
anti-inflammatory effects. Purpose: The aim of this study was to prove the effect of Nannochloropsis oculata extracts to decreased levels of TNF-α which induced by Actinobacillus actinomycetemcomitans bacteria. Materials and Methods: The experiment was held post test only control group design. 48 male wistar rats divided into 6 groups. K1 group was negative control group without any treatment, K2 group induced by Actinobacillus actinomycetemcomitans bacteria, K3 group treated with minocycline antibiotics 0.1%, and the other groups K4, K5, K6 were treated with Nannochloropsis oculata extract on various concentration that is 2.375%, 2.5%, 2.625%. After treatments on the 37th day, all groups of rats were euthanized and measured the levels of TNF-α in gingival tissue by ELISA. All of thedatas were analyzed by Kruskal Wallis dan Mann-Whitney U test. Result: This study showed the levels of TNF-α K2 group (36.9444) was significantly higher than K1 group(27.8889). K3 group (29.2778) showed no significant decreased compared to K2 group (36.9444). K4 (35.2778), K5 (33.1111) and K6 (29.2778)groups showed no significant decreased levels of TNF-α compared to K2 group (36.9444). Conclusion: Nannochloropsis oculata extracts can not reduce the levels of TNF-α in rats which induced by Actinobacillus actinomycetemcomitans bacteria.

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References

Macedo TCD, Costa MDCN, Filho ISG, Vianna MIP, Santos CT. 2006. Factors Related to Periodontal Disease in a Rural Population. Brazilian Oral ResearcH, 20(3): 88-74.

Tampubolon SN. 2005. Dampak Karies Gigi dan Penyakit Periodontal terhadap Kualitas Hidup. Pidato Pengukuhan. Fakultas Kedokteran Gigi Universitas Sumatera Utara, Medan, Indonesia. Available from http://repository.usu.ac.id/bitstream/123456789/205263/ppgb_2005_nurmala_situmorang.pdf.txt. Diakses 29 Oktober 2013.

Wahyukundari MA. 2009. Perbedaan Kadar Matrix Metalloproteinase-8 Setelah Scalling dan Pemberian Tetrasiklin pada Penderita Periodontitis Kronis. Jurnal PDGI, 58(1): 2-1.

Wangsarahardja K. 2005. Penyakit Periodontal Sebagai Faktor Risiko Penyakit Jantung Koroner. Universa Medicina, 24(3): 136.

Newman MG, Takei HH, Klokkevold PR, Carranza FA. 2012. Carranza’s Clinical Periodontology,11th ed., Philadelphia: WB Saunders Company. P. 494-492, 373, 201, 194, 44-41.

Susilowati, Imam M. 2011. Dinamika Ekspresi Gen Matrix Metalloproteinase-8 dan Tissue Inhibitor Metalloproteinase-1 pada Pemakaian Piranti Ortodontik Lepasan. Departement of Orthodontics, Fakultas Kedokteran Gigi Universitas Hasannudin Makassar, Indonesia. H. 9.

Available from https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&ved=0CCMQFjAA&url=http%3A%2F%2Frepository.unhas.ac.id%2Fbitstream%2Fhandle%2F123456789%2F606%2FMMP%2520dan%2520TIMP1%2520Medan.doc%3Fsequence%3D1&ei=gr0BU4u9E6SpiAflYHQBQ&usg=AFQjCNEH7EtLMlFrkLWOIj0RBT2xvYbITg&bvm=bv.61535280,d.aGc. Diakses 11 Januari 2014.

Grigoriodou ME, Spridon OK, Phoebus NM, Jorg RS. 2010. Interleukin-1 as a Genetic Marker for Periodontitis.Quintessence International, 41(6): 517.

Morimoto Y, Kawahara Kl, Tancharoen S, Kikuchi K, Matsuyama T, Hashiguchi. 2008. Tumor Necrosis Factor-α Stimulates Gingival Ephitelial Cells to Release High Mobility-Group Box.1.Journal of Periodontal Research,, 43(1) : 83-76.

Shapira L, Stabholz A, Rieckmann P, Kruse N. 2001. Genetic Polymorphism of the Tumor Necrosis Factor (TNF)-Alpha Promoter Region in Families with Localized Early-Onset Periodontitis. Journal Periodontal Research, 36(3): 186183.

Ejeil AL, Gaultier F, Tchen S, Senni K, Pellat B, Godeau G, Gogly B. 2003. AreCytokines Linked to Collagen Breakdown During Periodontal Tissue Progression?. Journal of Periodontology, 74(2): 201-196.

Schulz S, Machulla HK, Altermann W, Klapproth J, Zimmermann U, Glaser C, Kluttiq A, Stein J, Schaller HG, Reichert S. 2008. Genetic Markers of Tumour Necrosis Factor Alpha in Aggresive and Chronic Periodontitis. Journal of Clinical Periodontology, 35(6): 500-493.

Brook I. 2003. Microbiology and Management of Periodontal Infections. General Dentirtry, 51(5): 428-424.

Krismariono A. 2009. Antibiotika sistemik dalam perawatan penyakit periodontal. Periodontic Journal, 1(1): 1915.

Abuzar. 2012. Penentuan Konsentrasi Optimal Obat Kumur Minosiklin Dalam Rentang Konsentrasi 0.0125%-0.2% Terhadap

Bakteri Mixed Periodontopathogen. Skripsi, Fakultas Kedokteran Gigi Universitas Hang Tuah Surabaya, Indonesia. H. 3.

Yek EC, Cintan S, Topcuoglu N, Kulekci G, Issever H, Kantarci A. 2010. Afficacy of Amoxicilin and Metronidazole Combination for the Management ofGeneralized Aggressive Periodontitis. Journal of Periodontology. Vol 81(7): 974-964.

Williams RC. 2007. Host Modulation for the Treatment of Periodontal Disease. Inside Dentistry. Vol 3(1): 5-2.

Nassar CA, Nassar PO, Nassar PM, Spolidorio LC. 2005. Selective Cyclooxygenase-2 Inhibition Prevents Bone Resorption. Brazilian Oral

Research, 19(1): 40-36.

Tipton DA, Flin JC, Stein SH, Dabbous MKh, 2003. Cyclooxygenase-2 Inhibitors Decrease Interleukin- 1β– Stimulated Prostaglandin E2 and IL-6 Production by Human Gingival Fibroblasts. Journal of Periodontology,74(12): 1763-1754.

Mlachkova PA. 2009. Effectiveness of Additional Therapy with NSAID (aulin) on Distribution of Shallow and Periodontal Pockets in Patients with Chronic Periodontitis. Journal of IMAB Vol 15(2): 55.

Widjojo J. 2003. Obat Antiinflmasi NonSteroid Sebagai Penyebab Terbanyak Perdarahan Saluran Cerna.MKB, 35(1):2-1.

Yanuhar U. 2009. Pengaruh Pemberian Bahan Aktif Ekstrak Nannochloropsis oculata terhadap Kadar Radikal Bebas pada Ikan Kerapu (Cromileptesaltivelis) yang Terinfeksi Bakteri Vibrio alginolyticu. Jurnal Ilmiah Perikanan dan Kelautan, 1(2): 191-185.

Yanuhar U, Maizar A, Irawan B, Nurdiani R. 2011. Eksplorasi dan pengembangan bahan aktif mikroalga laut (Nannochloropsis oculata) sebagai antibakteri Vibrio alginolyticus dan respons imun secara in vivo pada ikan kerapu. Humback grouper. Berk. Penelitian Hayati Edisi Khusus: 6C (1-5).

Singh S. 2012. Structural Modeling and Simulation Studies of Human Cyclooxygenase 1 (HsCOX1) with Antiinflammatory

Terpenoids: Implications in Drug Targeting and Designing. Pharmaceutica Analytica Acta, 3(1): 121.

Bruunsgaard H, Henrik EP, Bente KB, Kristiina N, Jari K, Jukka TS. 2003. Long-Term Combined Supplementations with α-Tocopherol and Vitamin C Have No Detectable Anti-inflammatory Effects in Healthy Men. The Journal of Noutrition, 133(4): 1172-1170.

Mukhija M, Sundriyal A. 2013. Phytoconsituents Responsible for Anti-43. Kurniawati A. 2005. Uji Aktivitas Anti Inflamasi Ekstrak Metanol

Graptophyllum griff pada Tikus Putih. Majalah Kedokteran Gigi Edisi Khusus Temu Ilmiah Nasional IV, 11-13: 170167.

Fitriyani A, Winarti L, Muslichah, Nuri. 2011. Uji Antiinflamasi Ekstrak Metanol Daun Sirih Merah (Piper crocatum Ruiz & Pav) pada Tikus Putih. Majalah Obat Tradisional, 16(1): 42-32.

Hertiani T, Pramono S, Supardjan. 2000. Uji Daya Antioksidan Senyawa Flavonoid Daun Plantogo major L. Majalah farmasi Indonesia, 11(4): 246234.

Ernest P. 2012. Pengaruh Kandungan Ion Nitrat Terhadap pertumbuhan Nannochloropsis Sp. Skripsi, Fakultas Teknik Kimia Universitas Indonesia Jakarta, Indonesia. Available from http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rjaved=0CCsQFjAA&url=http%3A%2F%2Flontar.ui.ac.id%2Ffile%3Ffile%3Ddigital%2F20308618-S%2B42592Pengaruh%2Bkandunganfull%2Btext.pdf&ei=olYIU4PrPMrtrQezuIGgCA&usg=AFQjCNFwPOkDByKCxFuUBZcC2LjqMBnig&bvm=bv.61725948,d.bmk. Diakses 10 juli 2013.

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Published

2021-12-01

How to Cite

Peggie Octavia Halim, Syamsulina Revianti, & Yoifah Rizka Wedarti. (2021). Pengaruh Pemberian Ekstrak Nannochloropsis oculata Terhadap Penurunan Kadar TNF-α pada Tikus yang Diinduksi Bakteri Actinobacillus actinomycetemcomitans . DENTA JURNAL KEDOKTERAN GIGI, 9(1), 101–112. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/212

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Vol. 9 No. 1 (2015): Februari

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