Aplikasi Gel Kitosan Berat Molekul Tinggi dan Rendah terhadap Ketebalan Epitel Mukosa pada Proses Penyembuhan Luka Pencabutan Gigi

Authors

  • Fransiska Amelia Hartono Sarjana Kedokteran Gigi Universitas Hang Tuah
  • Puguh Bayu Prabowo Ilmu Material dan Teknologi Kedokteran Gigi Universitas Hang Tuah
  • Syamsulina Revianti Biologi Oral Fakultas Kedokteran Gigi Universitas Hang Tuah

Keywords:

Chitosan, molecular weight, epithelial thickness, wound healing

Abstract

Background: Acceleration of wound healing after tooth extraction is the most important. One of the tissue repair in wound healing is the reepithelialization. Chitosan is a biomaterial that can be used to accelerate reepithelialization in the wound healing process. Purpose: The aim of this study was to prove the differences between application of chitosan gel high molecular weight and low molecular weight to accelerate the reepithelialization in socket healing after tooth extraction. Material and Methods: The experiment was held the post test only control group design used 24 male wistar rats divided into 3 group. K1 group was control group
which was extracted without any treatment, K2 group was extracted and applicated by low molecular weight of chitosan gel, K3 group was extracted and applicated by high molecular weight of chitosan gel. After treatments on the 7 day, all groups of rats were euthanized and the epithelial mucosal thickness was measured under light microscope magnificant 100x. All of the data were analyzed by one way ANOVA and LSD test. Result: This study showed the epithelial thickness of the K2 and K3 groups was significantly higher than K1 group, but there were not significantly different between K2 and K3 group. Conclusion: The effectivity of high molecular and low molecular weight chitosan gel to accelerate reepithelialization in socket healing are similar.

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References

Pedlar J. 2007. Oral and Maxillofacial Surgery 2 nd ed. United States of America: Elsevier Saunders. P. 1.

Topazian RG, Goldberg MH., Hupp JR. 2002. Oral and Maxillofacial Infections 4ed. United States of America: Elsevier Saunders. P. 1.

Muflih A. 2008. Distribusi dan Frekuensi Pasien dengan Gigi Tiruan Jembatan di Klinik Integrasi RSGMP FKG UI Periode

Skripsi, Universitas Indonesia. H. 31.

Kumar V, Abdul K. Abbas, Nelson F. 2005. Robbins and Cotran Pathologic Basis of Disease. 7th ed. Philadelphia: Elsevier Saunders. P. 114-107.

Velnar, Bailey T, Smrkolj V. 2009. The Wound Healing Process: an Overview of the Cellular and Molecular Mechanism. The Journal of Internasional Medical Research, 37: 1542-1528.

Nield J dan Wilmann D. 2003. Foundation of Periodontics for the Dental Hygienist. United States of America: Wilham & Walkins. P. 81-1.

Diegelmann RF dan Evans MC. 2004. Wound Healing: an Overview of Acute, Fibrotic and Delayed Healing. Frontiers in Bioscience, 9: 289-283.

Larjava H. 2012. Oral Wound Healing: Cell Biology and Clinical Management 1st ed. Willey-Blackwell. P. 108-81.

Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M. 2008. Growth Factors and Cytokines In Wound Healing. Wound Repair and Regeneration. P. 588-585.

Sugita P, Wukirsari T, Sjahriza A, Wahyono D. 2009. Kitosan: Sumber Biomaterial Masa Depan. Bogor: IPB Press. P. 45-28.

Pieper JS, Van Wachem PB, Van Luyn MJA. 2000. Attachment of Glycosaminoglycans to Collagenous Matrices Modulates the Tissue Response in rats. Biomaterials, 21(16): 1699–1689.

Chin L dan Halim AS. 2009. In Vitro Models In Biocompatibility Assessment For Biomedical-Grade Chitosan Derivatives In Wound Management. J. Molecular Science, 10(3): 1313-1300.

Baitukalova TA, Bogoslovskaia OA, Ol’khovskaia IP, Gluschenko NN, Ovsiannikova MN, Lopatin SA, Varlamov VP. 2005. Regenerating activity and antibacterial effect of low-molecular-weight chitosan. Izv Akad Nauk Ser Biol,6: 659-63.

Ahlam A. 2011. Formulasi Sediaan Gel Ekstrak Lidah Buaya (Aloe Vera (L.) Webb) dengan Gelling Agent Kitosan dan Uji Efek Penyembuhan Luka Bakar. Skripsi, Universitas Muhammadiyah Surakarta. P. 3-2.

Budianto B. 2013. Pengaruh Kitosan Gel 1% Yang Memiliki Berat Molekul Tinggi dan Rendah Terhadap Jumlah Sel Osteoblas Pada Proses Penyembuhan Luka Pencabutan Gigi. Skripsi, Universitas Hang Tuah, Surabaya. H. 3-1.

Ueno H, Mori T, Fujinaga T. 2001. Topical Formulation and Wound Healing Applications Of Chitosan. Advanced Drug Delivery Reviews, 52(2): 115-105.

Masuoka K., Ishihara M., Asazuma T.,Hattori H., Matsui T. 2005. The interaction of chitosan with fibroblast growth factor-2 and its protection from inactivation. Biomaterials, 26: 3284-3277.

Schultz GS, Ladwig G, Wysocki A. 2005. Extracellular matrix: review of its roles in acute and chronic wounds. P. 1. Available from http://www.worldwidewounds.com/2005/august/Schultz/Extrace-MatricAcute-Chronic-Wounds.html.Diakses 5 Februari 2014.

Astuti T. 2006. Efek derajat deasetilasi dan konsentrasi kitosan terhadap daya hambat Streptococcus mutan dan Candida albicans. Tesis, Fakultas Kedokteran Gigi Universitas Airlangga. P. 40-25.

Saputra HA. 2012. Perbandingan Kesembuhan Luka Episiotomi Dengan Luka Ruptur Perineum Tingkat 1-2 Pada Primigravida Di RSUP H. Adam Malik Medan. Tesis, Universitas Sumatera Utara.H. 1.

Dai TH, Tanaka M, Huang YY, Hamblin MR. 2011. Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects. Expert review of anti-infective therapy, 9(7): 879-857.

Krause F. 2002. Wound Healing. P. 1. Available from http://www.charite.de/klinphysio/bioinfo/ 3_k-pathophyfromm/05ws_skripten/Krause/webscript_krause.htm Diakses 23 Juli 2013.

Morris PJ, Malt RA. 1995. Oxford Textbook of Surgery. Sec. 1 Wound healing. New York-Oxford-Tokyo Oxford University Press.P. 60-56.24. Rochima E, Suhartono MT, Syah D, dan Sugiyono. 2007. Viskositas dan Berat Molekul Kitosan Hasil Reaksi Enzimatis Kitin Deasetilase

Isolat Bacillus Papandayan. Seminar Nasional dan Kongres Perhimpunan Ahli Teknologi Pangan Indonesia (PATPI), Bandung. P. 7

Tang ZX, Qian JQ. 2007. Use of chitosan gel for the purification of protein. Braz. Arch. Biol. Technol, 50 (2). P. 1.

Available from http://www.scielo.br/scielo.php?pid=S1516-89132007000200015&script=sci_arttext Diakses 7 Februari 2014.

Rukmini A. 2007. Regenerasi minyak goreng bekas dengan arang sekam menekan kerusakan organ tubuh. Seminar Nasional Teknologi, Yogyakarta. P. 9-1.

Kusmawati D. 2004. Bersahabat dengan hewan coba, 1st ed., Gadjah Mada University Press. H. 1.

Tang ZX, Shi LE, Qian JQ. 2007. Neutral lipase from aqueous solutions on chitosan nano-particles. Biochemical Engineering

Journal, 34(3): 223-217.

Alemdaroglu C, Degim Z, Celebi N, Zor F, Ozturk S, Erdogan D. 2006. An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns, 32: 327-319.

Tawi M. 2008. Proses Penyembuhan Luka. H. 1.Available from http://www.syehaceh.wordpress.com/2008/05/13/prosespenyembuhan-luka Diakses 15 Juni 2013.

Sonia TA, Sharma CP. 2011. Chitosan and its derivatives for drug delivery perspective. Adv Polym Svi 243: 54-23.

Semalty A. 2006. Mucoadhesive Polymers–A review. Available from http://www.pharmainfo.net/reviews/mucoadhesive-polymers-review. Diakses 3 Februari 2014.

Sigma-Aldrich. 2013. Chitosan. P. 1. Available from http://www.sigmaaldrich.com/catalog/product/aldrich/448869?lang~en&region~1D.

Diakses 14 Januari 2014.

Alsarra IA. 2009. Chitosan topical gel formulation in the management of burn wounds. International Journal of Biological Macromolecules.P. 21-16.

Chen MC, Mi FL, Liao ZX, Sung HW. 2011. Chitosan: its Applications in Drug Eluting Devices. Advances in Polymer Science, 243: 230-185.

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Published

2021-12-01

How to Cite

Fransiska Amelia Hartono, Puguh Bayu Prabowo, & Syamsulina Revianti. (2021). Aplikasi Gel Kitosan Berat Molekul Tinggi dan Rendah terhadap Ketebalan Epitel Mukosa pada Proses Penyembuhan Luka Pencabutan Gigi . DENTA JURNAL KEDOKTERAN GIGI, 9(1), 1–10. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/195

Issue

Vol. 9 No. 1 (2015): Februari

Section

Articles

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