The Difference Compressive Strength of Scaffold Combination Chitosan and Aloe vera Extracted Water and Ethanol

Authors

  • Dwi Ayu Trisnawati Sarjana Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Sularsih Ilmu Material Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Widaningsih Prostodonsia Fakultas Kedokteran Gigi Universitas Hang Tuah

Keywords:

Scaffold, chitosan, Aloe vera extracted water, Aloe vera extracted ethanol, compressive strength.

Abstract

Background: Scaffold can be use in bone regeneration, but it should have similarities characteristic to a natural bone such as compressive strength that supported the process of growth and development of bone. This research use scaffold that made from chitosan and Aloe vera. Chitosan well-known as osteoconductive material, meanwhile Aloe vera was osteoinductive material. Purpose: The aim of this research was to compare compressive strength scaffold combinations of chitosan and Aloe vera extracted water and ethanol. Materials and Methods: Research design using post test only control group design with 36 scaffolds divided into 6 groups. Group 1 was scaffold Aloe vera extracted water (AVa), group 2 was scaffold Aloe vera extracted ethanol (AVe), group 3 was scaffold combination chitosan and Aloe vera extracted water 1: 1 (k-AVa1), group 4 was the scaffold combination of chitosan and Aloe vera extracted ethanol 1: 1 (k-AVe1), group 5 was scaffold combination of chitosan and Aloe vera extracted water 1: 2 (k-AVa2), and group 6 was scaffold combination of chitosan and Aloe vera ethanol extract 1: 2 (k -AVe2). Compressive strength test used Universal Testing Machine (Shimadzu Autograph–AGS–1kNX and using software Trapezium X). Data were analyzed using One Way ANOVA and Games Howell test. Results: The result of compressive strength AVa=1.36 MPa, AVe=1.03 MPa, k-AVa1=0.27 MPa, k-AVe1=0.11 MPa, k-AVa2=0.38 MPa, and k-AVe2=0.29 MPa. All groups have significant differences except AVa & AVe and k-AVa1 & K-AVe2 (p>0,05). Conclusion: There was difference between compressive strength scaffold combinations of chitosan and Aloe vera extracted water and ethanol, and the most highest compressive strength is AVa.

 

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References

Aufan MR, Daulay AH, Indriani D, Nuruddin A, B. S. Purwasasmita BS. 2012. Sintesiss Scaffold Alginat-Kitosan-Karbonat Apatit sebagai Bone Graft Menggunakan Metode Freeze Drying. Jurnal Biofisika. 2012;8(1): 24-16.

Swain SK, Bhattacharyya S, Sarkar D. Fabrication of Porous Hydroxyapatite Scaffold Via Polyethylene Glycol-Polyvinyl Alcohol Hydrogel State. Materials Research Bulletin. 2015. 64: 261-257.

Rodrigues CVM. Characterization of Bovine Collagen-Hydroxyapatite Composite Scaffold for Bone Tissue Engineering. Biomaterials. 2003;24(27): 4997-4987.

Shaik M & Subramanyam G. Stem Cell Scaffolds for Tissue Engineering. Juniper Publisher.2017;9(1):4-1.

Jagger DC, Harrison A. Denture Cleansing the Best Approach. Br Dent J. 1995;178: 413-7.

O’Brien FJ. Biomaterials & Scaffolds for Tissue Engineering. Materials Today. 2011;14(3):95-88.

Rahman S, Carter P, Bhattarai N. Aloe vera for Tissue Engineering Applications. Journal of Functional Biomaterials. 2017;8(1).

Xia W, Liu W, Cui L, Liu Y, Zhong W, Liu D, Wu J, Chua K, Cao Y. Tissue Engineering of Cartilage with the Use of Chitosan-Gelatin Complex Scaffolds. Journal of Biomedical Materials Research. Part B: Applied Biomaterials.2004; 71(2):380-373.

Suh FJK, Matthew HWT. Applications of Chitosan-based polysaccharide biomaterials in cartilage tissue engineering: A review. Biomaterials. 2000; 21(24): 2598-2589.

Surjushe A, Vasani R, Saple DG. 2008. Aloe vera: A short review. Indian Journal Dermato. 2008; 53(4): 166-163.

Suganya S, Venugopal J, Ramakrishna S, Laksmi BS, Dev VR, Giri. Aloe vera/Silk Fibroin/Hydroxyapatite Incorporated Electrospun Nanofibrous Scaffold for Enhanced Osteogenesis. Journal of Biomaterials and Tissue Engineering. 2014;4(1):19-9.

Cole L & Heard C. Skin Permeation Enhancement Potential of Aloe vera and a Proposed Mechanism of Action Based Upon Size Exclusion and Pull Effect. International Journal Pharmaceutics. 2007; 333 (6):10.

Rahmawati LA. Potensi Kombinasi Ekstrak Air dari Curcuma mangga Val., Acorus calamus L., dan Allium sativum Linn. Terhadap Aktivitas Antioksidan dan Antifungi Candida albicans Secara In-vitro. Skripsi. Surabaya: Fakultas Sains dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim; 2015. p. 29.

Carvalho RM, Mendonca JS, Santiago SL, Silveria RR, Garcia FC, Tay FR. Effect of HEMA/Solvent Combination Bond Strength to Dentin. Journal Dental Research. 2003;82(8):601-597.

Utomo S. Penggunaan Scaffold Kombinasi Kitosan, Aloe vera, dan Hidroksiapatit terhadap Jumlah Sel Osteoblas pada Penyembuhan Pencabutan Gigi Cavia Cobaya. Skripsi. Surabaya: Fakultas Kedokteran Gigi, Universitas Hang Tuah; 2018. p.29, 48.

Silva SS, Caridade SG, Mano JF, Reis RL. Effect of Crosslinking in Chitosan/Aloe vera Based Membranes for Biomedical Applications. Carbohydrate Polymers. 2013;98(1): 588-581.

Amalia F. Korelasi Antara Panjang Tulang Humerus dengan Tinggi Badan Pada Pria Dewasa Suku Lampung dan Suku Jawa di Desa Sukabumi Kecamatan Talang Padang Kabupaten Tanggamus. Karya Tulis Akhir. Lampung: Universitas Lampung; 2015. p. 9-10.

Chen G, Li W, Zhao B, Sun K. A Novel Biphasic Bone Scaffold: Beta Calcium Phospate & Amorpous Calcium Polyphosphate. Journal of the American Ceramic Society. 2009;92(4): 948-945.

Poinern GEJ, Brundavanam RK, Fawcett D. Nanometre Scale Hydroxyapatite Ceramics for Bone Tissue Engineering. American Journal of Biomedical Engineering. 2013; 3(6): 168-148.

Sudibyo. Metodologi Penelitian Aplikasi Penelitian Bidang Kesehatan. Surabaya: Unesa University Press: 2009. p. 19-20.

Wang Y, Wang K, Li X, We Q, Chai W, Wang S & Che Y. 3D Fabrication and Characterization of Phosporic Acid Scaffold with a HA/β-TCP Weight Ratio of 60:40 for Bone Tissue Engineering Applications. Journal Plos. 2017; 12(4):7.

Husseinsyah S & Mostapha M. The Effect of Filler Content on Properties of Coconut Shell Filled Polyester Composites. Malaysian Polymer Journal. 2011; 6(1):97-87.

Sinala S. Farmasi fisik. Bahan Ajar Cetak Farmasi. Kementerian Kesehatan Republik Indonesia. 2016. p.45.

Lay A. Formulasi Sediaan Gel Kombinasi Kitosan yang Memiliki Berat Molekul Tinggi dan Rendah dengan Ekstrak Gel Lidah Buaya (Aloe vera) Sesuai Kriteria Aplikasi di Rongga Mulut (Penelitian Observasional Analitik). Skripsi. Sjurabaya: Fakultas Kedokteran Gigi, Universitas Hang Tuah; 2016. p. 62-64.

Muhandri T. Pengaruh Ukuran Partikel, Kadar Padatan, Nacl Dan Na2co3 Terhadap Sifat Amilografi Tepung Dan Pati Jagung [The Effect of Particle Size, Solid Content, NaCl and Na2CO3 on The Amilographic Characteristics of Corn Flour and Corn Starch. Jurnal Teknologi dan Industri Pangan. 2017;18(2): 117-109.

Salim S, Rostiny, Mefina K. Efek Kombinasi Spirulina Kitosan untuk Preservasi Soket terhadap Osteoblas, Osteoklas, dan Kepadatan Kolagen. Dentika Dental Journal. 2015; 18(3): 231-225.

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Published

2021-08-12

How to Cite

Dwi Ayu Trisnawati, Sularsih, & Widaningsih. (2021). The Difference Compressive Strength of Scaffold Combination Chitosan and Aloe vera Extracted Water and Ethanol. DENTA JURNAL KEDOKTERAN GIGI, 13(1), 11–16. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/53

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