Innovation of Hyperbaric Oxygen Therapy and Stichopus hermanii to The Amount of Macrophages in Periodontal Ligaments in Pressure and Tension Areas During Orthodontic Tooth Movement

Authors

  • Jessica Jenuary Yasin Mahasiswa Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Arya Brahmanta Departemen Ortodonsia Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Agni Febrina Pargaputri Departemen Biologi Oral Fakultas Kedokteran Gigi Universitas Hang Tuah

Keywords:

Orthodontic treatment, HBOT, Stichopus hermanii, macrophages.

Abstract

Background: The prevalence of malocclusion in Indonesia reaches 80%, causing orthodontic treatment needs to increase. Orthodontic treatment takes a long time around 15-24 months so additional therapy is needed to accellerate the orthodontic treatment process. Hyperbaric oxygen therapy (HBOT) and Stichopus hermanii (golden sea cucumber) which contain bioactive ingredients can work synergistically to reduce inflammatory cytokines. Purpose: To determine the effect of  HBOT and Stichopus hermanii on the number of macrophages in periodontal ligament of pressure and tension area in the process of orthodontic tooth movement. Material and Methods: This research used Randomized Post Test Only Control Group Design. A sample of 30 marmot was randomly divided into 5 groups: negative groups (K-), positive groups (K+), Stichopus hermanii treatment group (P1), HBOT group (P2), and combination (P3). On K+ and treatment groups, maxillary incisors of marmot is installed helical spring in order to move the teeth to distal. Analysis of the number of macrophages  histologically using Hematoxylin Eosin (HE) staining. And then tested using the Kruskal-Wallis test, and the Mann Whitney Wilcoxon test. Result: The research data showed a decrease of mean average number of macrophages in the pressure area, K (-): 1.90 ± 0.41; K (+): 4.40 ± 1.14; P1: 4,30 ± 0,90; P2: 3.60 ± 0.54; P3: 2.90 ± 0.41 and the mean number of machrophages in tension area also descreased, K (-): 2.00 ± 0.35; K (+): 4.00 ± 1.00; P1: 4,30 ± 0,27; P2: 4,10 ± 0,65; P3: 3.60 ± 0.65. Conclusion: The combination of HBOT and Stichopus hermanii decreases the number of macrophages in the pressure and tension area in orthodontic tooth movement.

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References

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Published

2021-08-12

How to Cite

Jessica Jenuary Yasin, Arya Brahmanta, & Agni Febrina Pargaputri. (2021). Innovation of Hyperbaric Oxygen Therapy and Stichopus hermanii to The Amount of Macrophages in Periodontal Ligaments in Pressure and Tension Areas During Orthodontic Tooth Movement. DENTA JURNAL KEDOKTERAN GIGI, 13(1), 1–10. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/52

Issue

Vol. 13 No. 1 (2019): Februari

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