The Effect of Golden Sea cucumber (Stichopus hermanii) and Hyperbaric Oxygen Therapy to The Expression of Osteoprotegerin in Diabetes Mellitus Induce by Porphyromonas gingivalis Bacteria

Authors

  • Luthvina Setiami Aziza Undergraduate Faculty of Dentistry Hang Tuah University Surabaya
  • Kristanti Parisihni Biologi oral Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Dian Mulawarmanti Biologi oral Fakultas Kedokteran Gigi Universitas Hang Tuah

Keywords:

Diabetes Melitus, Periodontitis, Alveolar Bone Resorption, Osteoprotegerin, Immunohistochemical, Stichopus hermanii, hyperbaric Oxygen .

Abstract

Background: Diabetes mellitus (DM) with Periodontitis cause severe  alveolar bone resorption. Osteoprotegerin prevent osteoclastogenesis process so that inhibited alveolar bone resorption. Stichopus hermanii powder and hyperbaric oxygen therapy (HBO) have  content  and good effects in wound healing. Purpose: The aim of this reseach was to analyze the effect of Stichopus hermanii powder and HBO therapy  in  the increasing of osteoprotegerin expression on DM with periodontitis. Materials and Methods: The research was an experimental laboratories post test only control group design.  Twenty  Wistar rats were divided into 5 groups. K0 was negative group while  K1 was positive group K1-K4 groups were induced with 65mm/kg STZ single dose and 2ml bacterium P. gingivalis., K2 was treated with 3%golden sea cucumber powder in gel form. K3 was treated with OHB therapy 2.4 ATA for 7 days and K4 given a combination of both. Osteoprotegrin expression on osteoblast of alveolar mandible bone were examined by immunohistochemical staining. Data were analyzed by Kruskal-Wallis and Mann-Whitney test. Result: Kruskal-Wallis result showed significant differences from each treatment group (p <0.05). Mann-Whitney test showed the decreasion in the expression of osteoprotegerin between K1(2:50 ± 0.577) compare with K0 (p<0.05). Golden sea cucumber powder, HBO therapy and both combination had  increased osteoprotegerin expression.significancy in K2(8.25 ± 1.258), K3 (5.75 ± 0.957) and K4(12.50 ± 2.082) (p<0,05). Conclusion: Stichopus hermanii powder 3% and 2.4 ATA OHB therapy for 7 days increased  the expression osteoprotegrin on DM with periodontitis.

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Published

2021-09-14

How to Cite

Luthvina Setiami Aziza, Kristanti Parisihni, & Dian Mulawarmanti. (2021). The Effect of Golden Sea cucumber (Stichopus hermanii) and Hyperbaric Oxygen Therapy to The Expression of Osteoprotegerin in Diabetes Mellitus Induce by Porphyromonas gingivalis Bacteria. Denta Jurnal Kedokteran Gigi, 10(2), 165–174. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/138