Combination Administration of Stichopus hermaniiGel and Hyperbaric Oxygen Therapy the number of fibroblasts in diabetes mellitus rats with periodontitis

Authors

  • Faraziza Maulana Sarjana Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Dian Mulawarmanti Biologi Oral Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Fanny M.Laihad Bedah Mulut Fakultas Kedokteran Gigi Universitas Hang Tuah

Keywords:

Stichopus hermanii, hyperbaric oxygen, diabetes, periodontitis, fibroblasts

Abstract

Background: Periodontitis is the most severe complication in oral cavity to diabetes mellitus. Stichopus hermanii contain GAG, a polysaccharide which very useful in wound healing. Hyperbaric oxygen therapy is effective repairing ischemia, hypoxia, and is used as adjuvant therapy. Purpose: To determine effect of administration of Stichopus hermanii (SH) 3% gel and hyperbaric oxygen therapy 3x30 minutes with 5 minute intervals in 7 consecutive days to the number of fibroblasts in ligament periodontal diabetes mellitus rats with periodontitis. Materials and Methods: The experiment design is factorial design. Rats are inducted with STZ single dose 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis. 20 male wistar rats are divided into 5 groups. K- (control), K1 (STZ+P.Gingivalis), K2(STZ+P.Gingivalis+SH), K3(STZ+P.Gingivalis+HBO), K4(STZ+P.Gingivalis+SH+HBO). After 52 days treatment, rats is terminated to make a HPA Sample from the mandible and fibroblast is counted with 400x magnification. Result: One-way Annova here were significant differences (p<0,05) in K- (32.300 ± 11.381), K1 (31.725 ± 5.590), K2 (54.225 ± 7.165), K3 (49.725 ± 10.088), K4 (72.075 ± 9.342). Conclusion: administration of Stichopus hermanii 3% gel combination and 2.4 ATA hyperbaric oxygen 3x30 minutes a day with 5 minutes intervals in 7 consecutive days can increase the number of fibroblasts in diabetes mellitus rats with periodontitis.

 

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Published

2021-08-16

How to Cite

Faraziza Maulana, Dian Mulawarmanti, & Fanny M.Laihad. (2021). Combination Administration of Stichopus hermaniiGel and Hyperbaric Oxygen Therapy the number of fibroblasts in diabetes mellitus rats with periodontitis. DENTA JURNAL KEDOKTERAN GIGI, 11(2), 9–17. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/87

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Vol. 11 No. 2 (2017): Agustus

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