Efektivitas Kombinasi Grafting Cangkang Kerang Darah (Anadara granosa) dan Minyak Ikan Lemuru (Sardinella longiceps) Terhadap Penurunan Jumlah Osteoklas Pada Proses Bone Repair

Authors

  • Dinda Divilia Sarjana Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Hang Tuah Surabaya
  • Rima Parwati Sari Biologi Oral Fakultas Kedokteran Gigi Universitas Hang Tuah Surabaya
  • Paulus Budi Teguh Prostodonsia Fakultas Kedokteran Gigi Universitas Hang Tuah Surabaya

Keywords:

Blood cockle shell, sardine oil, osteoclasts, bone repair

Abstract

Background: In dentistry, complication post-extraction, disease periodontal, prosthetic surgery, pathological conditions, and trauma can cause alveolar bone loss and even lead to tooth loss. The use of hydroxyapatite derived from blood cockle shells is osteokondiction. Omega-3 in sardine oil will stimulate the healing process of damaged bone with decreasing in the number of the osteoclasts. Purpose: The aimed of this study to determine the Effectivity of Grafting Combination Blood Cockle Shell and Sardine Fish Oil to Decrease in The Numberof Osteoclasts in The Process of Bone Repair Methods: Animals used in research, as many as
24 male Wistar rats, which then divided into 4 groups, namely the control group and the treatment group. The control group subdivided into two groups, normal healing and human application's paste. In the treatment group, also divided into two groups, group with blood cockle shell application and combination of blood cockle shell and sardine oil. Osteoclasts viewed using HPA on the 7th day. Results: Decrease in The Number of Osteoclasts on K- group : 2.67±1,033, P1 : 2.33±0.09, K+ : 1.5±0.09, P2 : 0.83±0.54. Because the data not homogeneous then used test Kruskal-Wallis test and Mann-Whitney. P1 with P2 have a difference significantly compared 0,048 , P2 with K- 0,026 (p<0,05). Conclusions: Combined  administration of blood cockle shell (Anadara granosa) and sardine oil (Sardinella longiceps) significantly influence the amount of osteoclasts in the process of bone repair on the 7th day.

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References

Ferdiansyah, Rushadi D, Rantam F A, Aulani’am. 2011. Regenerasi pada Massive Bone Defect dengan Bovine Hydroxyapatite sebagai Scaffold Mesenchymal Stem Cell. JBP, 13(3):195-179.

Matoses VF, Vinarta MM, Domingo TA,Matoses IF, Liacer VJF. 2015. Treatment of multiple traumatized anterior teeth associated with an alveolar bone fracture in a 20-year-old patient: A 3-year follow up. J Clin Exp Dent, (4): e425-9.

Matthew P, William V, Caroline S, Zhu Z, Tejeda K. 2001. Surgical aids to Prosthodontics, Including Osseintegrated Implant in Pedlar J., Oral and Mxillofacial Surgery. Edinberg. Churchill Livingstone. P. 1.

Cochran DL.2008. Inflammation and bone loss in periodontal disease. J Periodontal, 79(8): 1569-76.

Little N, Rogers B, Flannery M. 2011. Bone Formation, Remodelling and Healing. Basic Science Surgery, 29(4):145-141.

Yustina, Ade Ratri. 2012. Peningkatan Periapikal Akibat Induksi Lipopolisakarida Porphyromonas Gingivalis (Suatu Penelitian Laboratories

menggunakan Tikus). Universitas Airlangga. H. 1.

Irawati L. 2014. Hubungan Tumor Necrosis Factor-Alfa (Tnf-A) dengan Kadar Hemoglobin dan Parasitemia pada Infeksi Malaria Falciparum. Disertasi Universitas Andalas. H. 99.

Hardani PR, Lastianny SP, Herawati D. 2013. Pengaruh Penambalan platelet-rich plasma pada Cangkok Tulang terhadap Kadar Osteocalcin Cairan Sulkus Gingiva pada Terapi Poket Infraboni. Jurnal PDGI, 62: 82-75.

Dumitrescu AL. 2011. Chemicals in Surgical Periodontal Therapy 2011th. Springer. P. 1.

Siregar NH. 2009, Keramik Sebagai Bahan Substitusi Bone Graft. Skripsi. Universitas Sumatra Utara. H. 1.

Ermiza, Linda. 2012. Perbandingan Dosis Akhir Sterilisasi 15 kGy dan 25 kGy bahan Allobatan (DFDB) Pada Proses Regenerasi Tulang. Tesis. Universitas Indonesia. H. 1.

Wu YL. 2007. Interaction of Bone Cells with Biomimetic Hydroxyapatite Gelatin P. 1.13. Nanocomposites Towards Developing

Bone Tissue Engineering. Thesis, University of Minnesota. P. 1.

Newmann MG,Takei HH, Carranza FA. 2011. Clinical Periodontology 11th edition. Philadelphia: Elsevier Saunders. p.672-678.

Awang-Hamzi AJ, Zuki ABS, Noordin MM, Jalila A, Norimah Y, 2007.Mineral Composition of the Cockle (Anadara

granosa) Shells of West Coast of Peninsular Malaysia and It’s Potential as Biomaterial for Use in Bone Repair. Journal of Animal and Veterianry

Advances 6(5).p.591-594

Hafisko H, Ardiyanto, Trixi M, 2013. Pemanfaatan Limbah Cangkang Kerang Darah (Anadara granosa Linn) Dalam Sintesis Nanohidroksiapatit sebagai Bone Implan untuk Kerusakan Tulang. H. 1.

Setyono DED, 2006. Karakteristik Biologi dan Produk Kekerangan Laut. Oseana, Volume XXXI No.1,2006.

Mahrus, Sumitro SB, Widodo N, Sartimbul A. 2012. The Association Between Genetic Variations and Omega3 Production on Sadinella lemuru in Lombok Strait. Journal of Agriculture and Veterinary Science.

Collins N and Suleweski C, 2011. Omega-3 Fatty Acid and Wound Healing. P. 1013.

Omar MN, Ahlam NS, Zainuddin NA, Yunus K, 2010. Ω-Fatty acid from malaysian giant snakehead (Channa micropeltes) fish oil. Oriental Jurnal of Chemistry,26(1).

Sulatani M, Stringer AM, Bowen JM, Gibson RJ. 2012. Anti-Inflamatory Cytokines : Important Immunoregulatory Factors Contributing to Chemotherapy Induced Gastrointestinal Mucositis. Chemotherapy Research and Practie. Vol 2012.

Asakura T, Nagano A, Tanioka Y, Sakurai N, Sezutsu H, Kiba H. 2011. Bone Regeneration on The Epicondyle of The Femur

Supported by Silk Fibroin-based Scaffold: A Model System for Dental Surgery. Journal of Insect Biotechnology and Sericoloy, 80: 25-3.

Sudibyo. 2013. Metodologi Penelitian Aplikasi Penelitian Bidang Kesehatan (Edisi Revisi), Buku 1. Surabaya: Unesa

University Press. H. 129-30, 20-19.

Tanideh N, Nazhvani SD, Jaberi FM, Mehrabani D, Rezazadeh S, PakbazS, et al. 2011. The Healing Effect of Bioglue in Articular Cartilage Defect of Femoral Candyle in Experimental Rabbit Model.Iranian Red CrescentMedical Journal, 13: 636-629.

Fleckhell P. 2009. Laboratory Animal Anastesia Edisi Ketiga. Elsevier. H. 187.

Rokn AR, Khodadoostan MA, Ghahroudin AARR, Motahhary P, Fard MJK, Bruyn HD, Afzalifar R, Soolar E, Soolar A. 2011. Bone Formation with Two Types of Grafting Materials : A Histologic and Histomorphometric Study. Open Dent. Journal. P. 104-96.

Kopschina MI, Marinowic DR, Klein CP, Araujo CA, Freitas TA, Hoff G,and Silva JB. 2012. Effectof Bone Marrow Mononuclear Cells Plus Platelet-rich Plasma in Femoral Bone Repair in Rats. Braz J. Vet. Res. Anim. Sci, Sao Paulo, 49(3): 184-179.

Schmidlin PR, Nicholls F, Kruse A, Zwahlen RA, Weber FE. 2011. Evaluation of In Situ Hardening Calcium Phosphate Bone Graft Substitutes. Clin Oral Implants Res, 24(2): 157-149.

Nanci A. 2008. Ten’s Cate Oral Histology Development Structurer and Function, edisi 7. St. Louis: Mosby Elsevier. P. 383-379, 77-57, 5.

Rasyid A. 2008. Isolasi Asam Lemak tak Jenuh Majemuk Omega-3 dari Ikan Lemuru (Sardinella longiceps). ProsidingSeminar Iptek Kelautan Nasional. Jakarta: Pusat Penelitian Oseanografi LIPI.

Riani. 2012. Evaluasi Radiografis Tinggi dan Densitas Tulang Alveolar pada Terapi Periodontitis Dengan Allograft (DFDBA) Dibandingkan Xenograft. Tesis, Universitas Indonesia: Jakarata. H. 17-15,12-10.

Awang-Hamzi AJ, Zuki ABS, Noordin MM, Jalila A, Norimah Y, 2007.Mineral Composition of the Cockle (Anadara granosa) Shells of West Coast of Peninsular Malaysia and It’s Potential as Biomaterial for Use in Bone Repair. Journal of Animal and Veterianry Advances 6(5): 594-591.

Marie B E N. 2003. The immune system innate and adaptive body defenses. Humans anatomy and physiology. New York : Benjamin Cummings. Boyce BF and Xing L. 2007. Biology of RANK, RANKL and Osteoprotegerin. Arthritis Research and Therapy. (9): 7-1.

Estiasih T. 2009, Minyak Ikan Teknologi dan Penerapannya untuk Pangan dan Kesehatan. Yogyakarta: Graha Ilmu.

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Published

2021-12-01

How to Cite

Dinda Divilia, Rima Parwati Sari, & Paulus Budi Teguh. (2021). Efektivitas Kombinasi Grafting Cangkang Kerang Darah (Anadara granosa) dan Minyak Ikan Lemuru (Sardinella longiceps) Terhadap Penurunan Jumlah Osteoklas Pada Proses Bone Repair . DENTA JURNAL KEDOKTERAN GIGI, 9(1), 20–29. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/197

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Vol. 9 No. 1 (2015): Februari

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