The Inhibition Effect of Green Microalga Ethanol Extract (Nannochloropsis Oculata) on The Number of Bacteria Streptococcus Mutans (In Vitro)
DOI:
https://doi.org/10.30649/denta.v15i1.7Keywords:
Antibacterial, nannochloropsis oculata, streptococcus mutansAbstract
Background: Antibacterial is a substance produced by a microorganism, which has the ability to inhibit growth or kill other microorganisms. Antibacterial activity is measured in vitro to determine the potency of an antibacterial agent in a solution, its concentration in body fluids or tissues, and the susceptibility of certain microorganisms to certain concentrations of drugs. Purpose: The purpose of this research was to find the power of extract ethanol Nannocholoropsis oculata as an alternative antibacterial causes of the growth of the caries which is Streptococcus mutans. Material and methods: extract ethanol. Nannocholoropsis oculate divided into three parts of concentration 1,5%, 2% and 2,5%, Chlorhexidine 0,12% as a control, culture bacteri Streptococcus mutans with McFarland 0.5 standart. The metode used in this study was One way analysis of varians (ANOVA), but first it had to be a homogenius test and a normalitas test. Result: The result of this study showed that no barrier power at concentration 1,5%, 2% and 2,5%. Conclucion: The result showed no inhibition in the three observed groups of 1.5%, 2%, and 2.5% of extract ethanol Nannochloropsis oculate. This meant that this article cannot prove the inhibition characteristic of Nannochloropsis oculate. This may be because the concentration of the extract Nannochloropsis oculata used in this study was too small. Henceforth, it is necessary to conduct research with a higher concentration of extract. On the other hand, the positive control group, using 0.12% Chlorhexidine solution, showed an inhibition characteristic while the negative control group, using 1 % of DMSO showed no inhibition.
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References
Kusumawardani C, Leman MA, & Mintjelungan CN. Pengaruh air kelapa terhadap peningkatan pH saliva. e-GiGi. 2017; 5(1).
Lely, MA. Pengaruh (pH) saliva terhadap terjadinya karies gigi pada anak usia prasekolah. Indonesian Bulletin of Health Research. 2017; 45(4): 241-248.
Yadav K, Prakash S. Dental caries: A Microbiological approach. J Clin Infectious Diseases & Practice. 2017; 2(1):1-15. DOI: 10.4172/2476-213X.1000118.
Rao, Arathi. Principles and Practice of Pedodontic. Jaypee Brothers Medical Publisher (P) Ltd: New Delhi; 2012.
Isnarianti, Rina, Ivan A, Wahyudi, Rini M. Puspita. Muntingia calabura L. Leaves Extract Inhibits Glucosyltransferase Activity of Streptococcus mutans. Journal of Dentistry Indonesia. 2013; 20(3): 59- 63.
Andries, Juvensius R, Gunawan, Paulina N, Supit, Aurelia. Uji Efek Anti Bakteri Ekstrak Bunga Cengkeh Terhadap Bakteri Streptococcus Mutans Secara In Vitro. e-GiGi. 2014; 2(2).
Biondi, N., and M. Tredici. Algae and Aquatic Biomass for a Sustainable Production of 2nd Generation Biofuels. 2011 ;148-150
Kagan ML, Matulka RA. Safety Assessment of The Microalgae Nannochloropsis oculata. Toxicol Rep. 2015; 2: 617-623.
Sathasivam R, Radhakrishnan R, Hashem A, Abd_Allah EF. Microalgae Metabolites: A Rich Source for Food and Medicine. Saudi J Biol Sci. 2019; 26(4): 709-722.
Fadhilah A. Daya Hambat Ekstrak Nannochloropsis oculata Terhadap Pertumbuhan Bakteri Enterococcus faecalis [skripsi]. Surabaya: Fakultas Kedokteran Gigi Universitas Hang Tuah; 2013. h. 56.
Yanuhar U, Maizar A, Irawan B, & Nurdiani R. Eksplorasi dan Pengembangan Bahan Aktif Mikroalga Laut (Nannochloropsis oculata) Sebagai Antibakteri Vibrio alginolyticus dan Respons Imun Secara In Vivo Pada Ikan Kerapu. Humback grouper. Berk. Penelitian Hayati Edisi Khusus C. 2011; (6): 1-5.
Kafaie S, Loh SP, Muhtarrudin N. Acute and Sub-chronic Toxicological Assessment of Nannochloropsis oculata in Rats. African Journal of Agricultural Research. 2012 ; 7(7): 1225-1220.
Nuno K, Villarruel-López A, Puebla-Pérez AM, Romero-Velarde E, Puebla-Mora AG, Ascencio F. Effects of The Marine Microalgae Isochrysis Galbana and Nannochloropsis Oculatain Diabetic Rats. J Functional Foods. 2013; 5(1): 106-115.
Sudibyo. Metodologi Penelitian. Buku 2. Unesa University Press: Surabaya; 2013. h.137.
K. Parisihni, S. Revianti. Antifungal effect of Sticophus hermanii and Holothuria atra Extract and Its Cytotoxicity on Gingiva-derived Mesenchymal Stem Cell. Dental Journal: Majalah Kedokteran Gigi. 2013; 46(4) : 218-23.
Saleh MR. Perbandingan Kadar Glikosaminoglikan dan Triterpene Glycoside pada Ekstrak Teripang Emas (Stichopus hermanii) dengan Pelarut Etanol (Polar) dan Heksana (Non polar) [skripsi]. Surabaya: Fakultas Kedokteran Gigi Universitas Hang Tuah; 2013.
Bordbar S. High-Value Components and Bioactives from Sea Cucumbers for Functional Foods – A Review. Marine Drugs. 2011: 1761-1805.
Marnoto T. Ekstraksi Tannin sebagai Bahan Pewarna Alami dari Tanaman Putrimalu (Mimosa pudica) Menggunakan Pelarut Organik. Reaktor. 2012; 14(1): 45-39.
Adrianto WDA. Uji Daya Antibakteri Ekstrak Daun Salam (Eugenia polyantha Wight) dalam Pasta Gigi Terhadap Pertumbuhan Streptococcus mutans [skripsi]. Jember: Fakultas Kedokteran Gigi Universitas Jember; 2012.
Gartika Meirina, and Satari MH. Beberapa Bahan Alam Sebagai Alternatif Bahan Pencegah Karies. 2013; Available from: pustaka. unpad. ac. id/wp_content/uplads/2, 13(08).
Torabinejad, Mahmoud, Ashraf Fouad, and Shahrokh Shabahang. Endodontics e-book: Principles and practice. Elsevier Health Sciences; 2020.
Alfath CR, Yulina V, Sunnati. Efek Antibakteri Ekstrak Kulit Buah Delima (Granati fructus cortex) pada Streptococcus mutans In Vitro. Journal of Dentistry Indonesia. 2013; 20 (1): 8.
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