Evaluation of Antibacterial Activity of Cymbopogon Nardus L. on The Growth of Enterococcus Faecalis

Authors

  • Rosada Sintya Dwi Department of Dentistry, Medical Faculty, University of Sriwijaya, Palembang, South Sumatera, Indonesia
  • Diah Tanjung Department of Dentistry, Medical Faculty, University of Sriwijaya, Palembang, South Sumatera, Indonesia
  • Shanty Chairani Department of Dentistry, Medical Faculty, University of Sriwijaya, Palembang, South Sumatera, Indonesia
  • Rini Bikarindrasari Department of Dentistry, Medical Faculty, University of Sriwijaya, Palembang, South Sumatera, Indonesia
  • Siti Rusdiana Puspa Dewi Department of Dentistry, Medical Faculty, University of Sriwijaya, Palembang, South Sumatera, Indonesia

DOI:

https://doi.org/10.30649/denta.v15i1.4

Keywords:

Antibacterial, Cymbopogon nardus L., Enterococcus faecalis, inhibition zone.

Abstract

Background: Cymbopogon nardus L. known as citronella grass is traditional medicinal plant that contains saponins, polyphenols, alkaloids, flavonoids, and essential oil. Based on the previous study, it is known that it has antibacterial properties. Objective: The aim of this study was to determine antibacterial activity of Cymbopogon nardus L. in inhibiting the growth of Enterococcus faecalis. Methods: The samples were Cymbopogon nardus L. extracts with concentration of 1%, 3%, 5%,7%, 9%, and 5% NaOCl as positive control. Antibacterial activity of citronella extract against E. faecalis was observed with the formation of inhibitory zone diameter in agar. Data were analyzed using SPSS with one-way ANOVA and LSD test. Result: The result showed that Cymbopogon nardus L. was able to inhibit the growth of E. faecalis at concentration 1%,3%,5%,7% and 9%. There was a significant difference in antibacterial activity among all groups. It showed that concentration of 9% showed the highest zone of inhibition, but it was not as effective as NaOCl 5%. NaOCl showed the highest zone of inhibition. Conclusion: Cymbopogon nardus L. had antibacterial activity in inhibiting the growth of E. faecalis. The higher the concentration of citronella extract, the higher the antibacterial activity possessed.

Downloads

Download data is not yet available.

References

Purnamasari AD, Munadziroh E, Yogiartono MR. Konsentrasi ekstrak biji kakao sebagai material alam dalam menghambat Streptococcus mutans. Jurnal PDGI 2010;59(1):14-8.

Kardinan A. Tanaman penghasil minyak atsiri. Jakarta: Agromedia; 2005 :5-8.

Raharjoe JS, Windardi FI. Empat serangkai tanaman sebagai bahan aromatik pada suku Mongondow, Sulawesi Utara. Proseding Simposium Nasional 1 Tumbuhan Obat dan Aromatik APINMAP 1996 : 507.

Ilyas M. Daya hambat ekstrak buah mengkudu terhadap pertumbuhan Candida albicans. Dentofasial 2008; 7(1): 7-12.

Mabhiza D, Chitemerere, Mukangayama S. Antibacterial Properties of Alkaloid Extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. Int. J Med Chem. 2016; 2016: 1-7.

Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial Properties of Plant Essential Oils against Human Pathogens and Their Mode of Action: An Updated Review. Evid Based Complement. Alternat. Med. 2016; 2016: 3012462.

Supriyanto. Potensial ekstrak sereh wangi (Cymbopogen nardus L.) sebagai anti Streptococcus mutans. Skripsi. Bogor: Institut Pertanian Bogor; 2010.

Brugnera DF, Oliviera MM, Piccoli RH. Essential oils of Cymbopogen sp. in the control of foodborne pathogenic bacteria. Alim Nutr 2011; 22 (3): 339-43.

Lee W, Lim S, Son H, Bae K. Sonicated extract of Enterococcus faecalis induces irreversible cell cycle arrest in phytohemagglutinin-activated human lymphocytes. J Endod 2004;30:209 –12.

Santoso HB. Sereh wangi: bertanam dan penyuklingan. 5thed Yogyakarta : Kaninus; 2010:43.

Ukwubile C, Malgwi TS, Dibal MY, Aliyu N. Preliminary phytochemical screening and antimicrobial activity of Cymbopogon citratus (DC) Satpf. (Poaceae) leaf ethanol extract against selected microbes citation. Am J Microbiol Biotech. 2017; 4(5): 61-6.

Ismaili A, Sohrabi SM, Azadpour M, Heydari R, Rashidipour M. Evaluation of the activity of alkaloid extracts of four papaver species. Herb. Med. J. 2017; 2(4): 1-7.

Wang G, Vasquez KM. Effects of replication and transcription on DNA structure-related genetic instability. Genes. 2017; 8(1): 17.

Manosalva L, Mutis A, Urzua A, Fajardo V, Quiroz A. Antibacterial activity of alkaloid fraction from Berberis microphylla G. Forst and study of synergism with Ampicillin and Cephalothin. Molecules. 2016; 21: 76-86.

Ravi L, Manasvi V, Laksmi P. Antibacterial and antioxidant activity of saponin from Abution indicum leaves. Asian J. Pharm. Clin. Res. 2016; 9(3): 344-7.

Su Y, Ma L, Wen Y, Wang H, Zhang S. Studies of the in vitro antibacterial activities of several polyphenols against clinical isolates of methilcillin-resistant Staphylococcus aureus. Molecules 2014; 19: 12630-9.

Alghazeer R, Elmansori A, Sidati M, Gammoudi F, Azwai S, Naas H, Garbaj A, Eldaghayes I. In vitro antibacterial activity of flavonoid extracts of two selected Libyan algae against multi-drug resistant bacteria isolated from food products. J Biosci. Med. 2017; 5: 26-48.

Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some essential oils- present status and future perspectives. Medicines 2017; 4(3): 58.

Zand V, Lotfi M, Soroush MH, Mojadadi A. Antibacterial efficacy of different concentration of sodium hypoclorite gel and solution on Enterococcus faecalis. Iranian Endo. J. 2016; 11(4): 315-9.

Haapasalo, M., Shen, Y., Wang, Z. et al. Irrigation in endodontics. Br Dent J 216, 299–303 (2014). https://doi.org/10.1038/sj.bdj.2014.204

Downloads

Published

2021-08-13

How to Cite

Rosada Sintya Dwi, Diah Tanjung, Shanty Chairani, Rini Bikarindrasari, & Siti Rusdiana Puspa Dewi. (2021). Evaluation of Antibacterial Activity of Cymbopogon Nardus L. on The Growth of Enterococcus Faecalis. DENTA, 15(1), 24–29. https://doi.org/10.30649/denta.v15i1.4

Issue

Section

Articles

Most read articles by the same author(s)