The Influence of Ethanol Extract Graptophyllum pictum L. Griff toward Phagocytic Activity of Monocyte Exposed by Candida albicans

Authors

  • Atik Kurniawati Department of Oral Biology, Faculty of Dentistry, Jember University, Indonesia

Keywords:

Ethanol extract of Graptophyllum pictum L. Griff leaves, Monocyte’s Phagocytic Activity, Candida albicans

Abstract

Background: Candidiasis is one of oral infectious disease caused by Candida albicans.The role of C. albicans as pathogen opportunistic in oral infection can affected by immune system. Phagocytosis has contributed in immune system against C. albicans infection, which is the role of monocytes. Grapthophyllum L. Griff has been proved to increase macrophage’s phagocytic activity, but the effect of violet leaves ethanol extract (EEDU) to increase monocyte’s phagocytic activity on C. albicans is unknown. Objectives: To determine the effect of violet leaves extract on monocyte’s phagocytic activity on C. albicans. Methods: This research was acted in vitro on human monocyte cell culture. There was 5 groups: negative control group, positive control group (incubated in Isoprinosine) and treatment groups was incubated in EEDU 2,5%, 5%, 10%. The percentage of activated monocytes was counted. Result: The result showed that incubation of EEDU 2,5%, 5% and 10% could increase monocyte’s phagocytic activity significantly (p<0,05) compared to control groups. The number of monocyte’s phagocytosis on C. albicans incubated with EEDU 2,5%, 5% and 10% is 65%, 60,5%, and 58,75%. The optimal concentration of EEDU to increase monocyte’s phagocytic activity was 2,5%. Conclusion: The study showed that EEDU can increase monocyte’s phagocytic activity on C. albicans.

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References

Newman, Takei, Klokkevold, Carranza. Carranza’s clinical periodontology. 11th ed. St. Louis: Elsevier Inc; 2012.

Regezi JA, Sciubba JJ, Jordan RCK. Oral pathology: clinical pathologic correlations. 4th Edition. St. Louis: Saunders; 2003.

Jawetz, Melnick, Adelberg. Mikrobiologi kedokteran. Edisi 23. Jakarta: EGC; 2007.

Ratnawati H, Handoko Y, Purba LH. Pengaruh pemberian ekstrak buah merah (Pandanus conoideus Lam.) terhadap aktivitas fagositosis makrofag. JKM. 2007; 7(1):04-01.

Wahyukundari MA. Laporan hasil penelitian dosen pemula Aktivitas fagositosis neutrofil dan monosit yang dipapar ekstrak daun binahong. Jember: FKG UNEJ; 2013.

Ashman RB, Papadimitriu JM. Production and function of cytokines in natural and acquired immunity to Candida albicans infection. Microbiology Rev. 1995; 59 (4): 672-646.

Vázquez-Torres A, Balish A. Macrophages in Resistance to Candidiasis. Microbiol. Mol. Biol. Rev. 1997; 61(2):192-170.

Krewer, Suleiman, Duarte, Ribeiro, Mostardeiro, Montano, Rocha, Algarve, Bresciani, Cruz. Guarana, a supplement rich in caffein and catechin, modulates cytokines: evidence from human in vitro and in vivo protocols. Eur. Food Res. Tech nol. 2014; 239; 57-49.

Lyu SY, Park WB. Production f Cytokine and NO by RAW 264.7 Macrophages and PBMC In Vitro Incubation with Flavonoids. Arch Pharm Res. 2005; 28(5): 581-573.

Heyne, Atlas Tumbuhan Indonesia, Penebar Swadaya, Jakarta; 1986. h.6-34.

Kurniawati A, Ekspresi TLR-2, TNF-alpha,IFN-gamma, dan TGF-betha Paru Mencit terinfeksi Mycobacterium tuberculosis pada Perbaikan Jaringan Paru Mencit dengan Pemberian Ekstrak Metanol Daun Ungu (Graptophyllum pictum L. Griff), Disertasi, Fakultas Kedokteran, Universitas Airlangga, Surabaya; 2014.

Kusumawati I, Pengaruh ekstrak etanol daun ungu terhadap fungsi fagositosis serta pembentukan imunoglobulin M dan TNF alpha pada mencit, Disertasi, Fakultas Pasca Sarjana Universitas Airlangga Surabaya; 2002. h.32-64.

Dada-Olagbende, SO. SO Ogbonia, HAB Coker and GE Ukpo. Blood glucose lowering effect of aqueos extract of Graptophyllum pictum (Linn) Griff. On aloxan-induced diabetic rats and its acute toxicity in mice, African Journal of Biotechnology. 2011; 10(6): h.1039-43.

Zhao, Yang, Wang, Liu, Jian. Immunomodulatory and anticancer activities of flavonoids extracter from litchi (Litchi chinesis Sonn.) pericarp. International Immunopharmacology. 2007; 7: 166-162.

Asti SIP. Skripsi Pengaruh ekstrak biji kopi robusta (Coffea robusta) terhadap aktivitas fagositosis sel monosit. Jember: FKG UNEJ; 2015.

Natea, Brown, Kullberg, Gow. An integrated model of the recognition of Candida albicans by immune system. Microbiology. 2008; 6: h. 78-67.

Natea, Joosten, Meer, Kulberg, Veerdonk. Immune defence against Candida fungal infections. Immunolog ; 2015. h. 1-13.

Nayeem N, Denny G, Mehta SK. Comparative Phytochemical Analysis, Antimicrobial and Antioxidant Activity of The Methanolic Extracts of The Leaves of Coffea Arabica and Coffea Robusta. Der Pharmacia Lettre. 2005; 3 (1): 297-292.

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Published

2021-08-12

How to Cite

Atik Kurniawati. (2021). The Influence of Ethanol Extract Graptophyllum pictum L. Griff toward Phagocytic Activity of Monocyte Exposed by Candida albicans. DENTA JURNAL KEDOKTERAN GIGI, 12(2), 126–133. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/65

Issue

Vol. 12 No. 2 (2018): Agustus

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Articles

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