Antibacterial Activity Test of Ethyl Asetate Fraction of Gletang Growth (Tridax procumbens L.) on The Growth of Enterococcus faecalis

Authors

  • Widyawati Department of Conservative, Faculty of Dentistry, Baiturrahmah University, Padang, Indonesia
  • Ulima Audia Fayi Arta Student of the Faculty of Dentistry, Baiturrahmah University, Padang, Indonesia
  • Okmes Fadriyanti Department of Conservative, Faculty of Dentistry, Baiturrahmah University, Padang, Indonesia

DOI:

https://doi.org/10.30649/denta.v20i1.1

Keywords:

Enterococcus faecalis, inhibition zone, Tridax procumbens L.

Abstract

Background: Intractable root canal infection remains a serious obstacle in endodontic therapy and is a frequent cause of treatment failure, with Enterococcus faecalis (E. faecalis) as a key pathogen. This Gram-positive bacterium can persist under harsh conditions within the root canal system. Chlorhexidine is commonly used as a chemical irrigant, but its long-term application is associated with undesirable side effects, prompting the search for natural antibacterial alternatives. One promising candidate is the gletang plant (Tridax procumbens L.), which contains bioactive compounds with potential antimicrobial effects. Objective: This study aimed to evaluate the antibacterial activity of the ethyl acetate fraction of Tridax procumbens L. at various concentrations against E. faecalis. Method: A laboratory experimental design with post test only control group was used. Antibacterial testing was performed using the agar diffusion method with the gletang ethyl acetate fraction at 2%, 4%, 6%, and 8%, chlorhexidine as a positive control, and 96% methanol as a negative control. Data were analyzed univariately in tables and bivariately using Kruskal–Wallis and Mann–Whitney tests. Results: The fraction inhibited E. faecalis growth, with the largest mean inhibition zone of 3.6 mm at 8% and the smallest at 2%, 1.6 mm. Overall activity across all concentrations was classified as weak; however, statistical analysis (p=0.003, p<0.05) confirmed a significant inhibitory effect. Conclusion: The ethyl acetate fraction of the gletang plant (Tridax procumbens L.) at 8% is effective as an antibacterial against Enterococcus faecalis, with an average inhibition zone diameter close to that of the positive control. The higher the concentration of the ethyl acetate fraction, the higher the bacterial inhibition.

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References

Subrata A, Prahasti AE, Iskandar BO. Influence of two root canal obturation techniques with resin-based sealer to Enterococcus faecalis penetration. J Indones Dent Assoc. 2019;2(1):21. doi:10.32793/jida.v2i1.358.

Tamara R, Rochyani L, Budi Teguh P. Daya hambat ekstrak teripang emas (Stichopus hermanii) terhadap bakteri Enterococcus faecalis. 2015;9(1).

Sofiani E, Mareta AD. Perbedaan daya antibakteri antara klorheksidin diglukonat 2% dan ekstrak daun jambu biji (Psidium guajava Linn) berbagai konsentrasi terhadap Enterococcus faecalis. Insisiva Dent J. 2014;3(1):30-41.

Sayekti S, Subiwahyudi A, Prasetyo E. Perbedaan efektivitas daya antibakteri ekstrak daun mimba (Azadirachta indica A Juss) dibanding NaOCl 2,5% terhadap Enterococcus faecalis. Conserv Dent J. 2016;6:71-76. doi:10.20473/cdj.v6i2.2016.71-76.

Tonini R, Salvadori M, Audino E, Sauro S, Garo ML, Salgarello S. Irrigating solutions and activation methods used in clinical endodontics: a systematic review. Front Oral Health. 2022;3:838043.

Dea AHB, H EH, Susanto H. Uji efektivitas suspensi ekstrak daun songgolangit (Tridax procumbens L.) terhadap penurunan kadar asam urat pada tikus putih jantan. J Farm Sains. 2017;1(1):72-79.

Ranjini SS, Abinash A, Sampath S, Yuvaneka S, Samiappan SC. Tridax procumbens-mediated silver nanoparticles synthesis against biofilm-forming pyogenic bacteria associated with wound. 3 Biotech. 2026;16(2):66.

Ingole VV, Mhaske PC, Katade SR. Phytochemistry and pharmacological aspects of Tridax procumbens (L.): a systematic and comprehensive review. Phytomedicine Plus. 2022;2(1):100199.

Putri FE, Diharmi A, Karnila R. Identifikasi senyawa metabolit sekunder pada rumput laut coklat (Sargassum plagyophyllum) dengan metode fraksinasi. J Teknol Ind Pertan Indones. 2023;15(1):40-46. doi:10.17969/jtipi.v15i1.23318.

Imansyah MZ. Uji daya hambat salep ekstrak etanol daun pandan wangi (Pandanus amaryllifolius Roxb.) terhadap bakteri Staphylococcus aureus. J Kesehat Yamasi Makassar. 2022;6(2):42-49.

Sharma K, Kumar S, Pandey AK. Antibacterial activity of terpenoids in plant extracts: a review. J Ethnopharmacol. 2020;258:112874. doi:10.1016/j.jep.2020.112874.

Kumar S, Prasad A, Iyer S, Vaidya S. Pharmacognostical, phytochemical, and pharmacological review on Tridax procumbens Linn.

Salasa AM, Daswi DR, Arisanty A. Potensi antibakteri ekstrak etanol daun pegagan (Centella asiatica [L.] Urban) terhadap pertumbuhan Enterococcus faecalis dan Citrobacter freundii. Media Farm. 2022;18(1):67-73.

Delina S, Arina Y. Uji aktivitas antibakteri dari fraksi daun kedondong bangkok (Spondias dulcis Forst.). J Aisyiyah Med. 2022;7(2).

Nurfatimah DA, Anwar R, Hardhani PR. Activity of water and ethanol Syzygium myrtifolium Walp. as antibacterial against Aggregatibacter actinomycetemcomitans. DJKG. 2025;19(1):1-7.

Widyawati UA, Fadriyanti O, Situmeang B, Silaban S. Antibacterial activity test of different parts of gletang (Tridax procumbens) from West Sumatera, Indonesia. Rasayan J Chem. 2022;15(4):2382-2386.

Wijayanti S, Pradana AF, Situmeang B, Prastiwi DA, Musa WJ. Microemulsion of methanol extract of Tridax procumbens flower and its antibacterial activity against Streptococcus mutans and Enterococcus faecalis. J Beta Kim. 2025;5(1):56-61.

Situmeang B, Musa WJ, Bialangi N, Sriwijayanti S, Widiyanto H, Susvira D. Antioxidant and antibacterial properties of methanol extract of gletang flower (Tridax procumbens). Biol Med Nat Prod Chem. 2025;14(2):1085-1090.

Ambulkar S, Ambulkar P, Deshmukh MP, Budhrani AB. Various dosage forms of Tridax procumbens and their antimicrobial activity against specific pathogens. Indian J Forensic Med Toxicol. 2020;14(4):6585-6588.

Jangid T, Jain A, Bhardwaj GS, Jangir RN. A comprehensive review on traditional uses, phytochemical constituents, and pharmacological properties of Tridax procumbens. J Phytopharmacol. 2025;14(4):223-246.

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Published

2026-02-18

How to Cite

Widyawati, Ulima Audia Fayi Arta, & Okmes Fadriyanti. (2026). Antibacterial Activity Test of Ethyl Asetate Fraction of Gletang Growth (Tridax procumbens L.) on The Growth of Enterococcus faecalis. Denta: Jurnal Kedokteran Gigi, 20(1), 1–6. https://doi.org/10.30649/denta.v20i1.1

Issue

Vol. 20 No. 1 (2026): Februari

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