Effect of Nutmeg (Myristica Fragrans) Methanolic Extract to the Growth of Dental Plaque Bacteria

Authors

  • Dewi Rosmalia Dental Health Study Program, Health Polytechnic of the Ministry of Health Padang
  • Minarni Dental Health Study Program, Health Polytechnic of the Ministry of Health Padang
  • Mhd Riza Marjoni Department of Phytochemistry, Dwi Farma Pharmacy of Academy Bukittinggi

DOI:

https://doi.org/10.30649/denta.v16i2.3

Keywords:

Dental Plaque, Myristica Fragrans, Bacteria, Streptococcus mutans, Dental Caries

Abstract

Background: One of the causes of dental caries is Streptococcus mutant bacteria which has the ability to change the sugar content of food waste into lactic acid as the initial process of caries. One of the efforts to overcome dental caries is to use natural ingredients such as nutmeg. Every part of nutmeg has active substances that are efficacious as antimicrobial, antibacterial, antioxidant, antifungal and anti-inflammatory. Pulpam, seeds and fuli of nutmeg showed as potential extracts in inhibiting the growth of Streptococcus mutans. Objective: This study aimed to analyze methanol extracts from pericarpium, pulpam, fuli and nutmeg seeds against the growth of Streptococcus mutans bacteria that causes dental plaque. Methods: The research was conducted by diffusion method using BHI agar media and incubated anaerobically at 37°C for 24 hours. Results: The results of the calculation of the average diameter of the inhibition zones from the extract of fuli, seeds, pulpam and pericarpium of the nutmeg plant were 19.00 mm, 25.33 mm, 15.66 mm, 22.66 mm, and 21.83 mm, respectively. Data analysis using ANOVA (one way) showed that there were significant differences in all groups at p<0.05. The results of the LSD test showed that there were significant differences in all treatment groups. Conclusion: Methanol extract from fruit and fruit parts of nutmeg showed the ability to inhibit the growth of bacteria that cause dental plaque.

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References

The Ministry of Health. Basic Health Research. Indonesian Ministry of Health Research and Development. 2018;53(9).

Dewi R kumala, Firdaus IWAK, Hakim AQ. Empowerment of Kaporagi in Reducing the Incidence of Tooth Caries in Toddlers in Wetlands. Proceedings of the National Conference on Community Service and Corporate Social Responsibility (PKM-CSR). 2020; 3 .

Mujadi ATF. Inhibition of caries in pediatric patients. Evaluation of Fluorine Application Method (NaF) One Visit at the Dental Clinic Poltekkes Yogyakarta Ministry of Health on Caries Inhibition in Pediatric Patients, Journal of Oral Health Care. 2014; 1(1):.

Noviyandri PR, , N, Chismirina S. Effect of Nutmeg Flesh (Myristica fragrans Houtt) against Streptococcus mutans Growth. J Syiah Kuala Dent Soc. 2021; 5(1): 1-5.

Thomas A. Traditional Medicinal Plants. Yogyakarta: Kanisius Publishers; 2007.

Widodo Santoso A, Simamora A, Simamora A, Herawan Timotius K, Herawan Timotius K. Bioactivities of Water Extract and Essential Oil from the Mace of Myristica fragrans Houtt. J Kedokt Yars. 2018; 26(2).

Winarti C, Nurdjanah N. Opportunities for Spice and Medicinal Plants as a Source of Functional food. Journal of Agricultural Research and Development. 2005; 24(12): 47-55.

Hafif B. The Strategy to Maintain Indonesia as a Main Nutmeg Producer in the World. J Penelit dan Pengemb Pertan. 2021;40(1).

Statistics Indonesia West Sumatra. the Central Bureau of Statistics West Sumatera. 2016.

Al-Bataina BA, Maslat AO, Al-Kofahi MM. Element Analysis and Biological Studies on Ten Oriental Spices Using XRF and Ames Test. J Trace Elem Med Biol. 2003;17(2).

Panggabean KA, Rusmarilin H, Suryanto D. The Utilization of Nutmeg Seed (Myristica fragrans Houtt) Extract as an Antimicrobial on Tempeh Sausage. In: IOP Conference Series: Earth and Environmental Science. 2019.

Satria WP. Archipelago Herbal Book: Various Recipes & Medicinal Plants For Various Health Disorders. Kata Hati. Yogyakarta. 2015.

Gilani SJ, Taleuzzamana M, Jahangirb A. Quantification and Identification of Bioactive Eugenol in Myristica fragrans Seed Using Validated High Performance Thin Layer Chromatography Technique. Pharm Anal Acta. 2017; 8(10).

Guntur G, Harlia H, Sapar A. Identification of Essential Oil Components of Nutmeg Flesh (Myristica Fraghans Houtt.) from Lemukutan Island and Anti-Inflammatory Activity Test Using the Menbran Stabilization Method of RBCs (Red Blood Cells). Al-Kimia. 2019;7(2).

Gratia B, Yamlean PVY, Mansauda KLR. Toothpaste Formulation Ethanol Extract of Nutmeg (Myristica fragrans Houtt.) Pharmacon. 2021;10(3).

Rumopa PME, Awaloei H, Mambo C. Inhibition Test of Nutmeg Seed Extract (Myristicae fragrans) Against the Growth of Staphylococcus aureus and Streptococcus pyogenes Bacteria. Journal of e-Biomedicine. 2016; 4(2).

Ismiyarto I, Ngadiwiyana N, Mustika R. Isolation, Identification of Nutmeg Fuli Essential Oil (Myristica fragrans) and Activity Test as a Larvicide. Journal of Science and Application Chemistry. 2009; 12(1): 23-30.

Shafiei Z, Shuhairi NN, Md Fazly Shah Yap N, Harry Sibungkil CA, Latip J. Antibacterial Activity of Myristica Fragrans Against Oral Pathogens. Evidence-Based Complement Altern Med. 2012; 2012.

Setty J, Srinivasan I, Sathiesh R, Kale M, Shetty V, Venkatesh S. In vitro Evaluation of antimicrobial effect of Myristica fragrans on Common Endodontic Pathogens. J Indian Soc Pedod Prev Dent. 2020; 38(2).

Arrizqiyani T, Sonjaya N, Asty A. Optimizing the Potential of Nutmeg as Antibacterial Escherichia coli Using the Extraction Method. Proceedings of the National Seminar. 2017 September; 375-82.

Shan B, Cai YZ, Brooks JD, Corke H. The in Vitro Antibacterial Activity of Dietary Spice and Medicinal Herb Extracts. Int J Food Microbiol. 2007;117(1).

Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 2005; 26.

Ngo DH, Vo TS, Ngo DN, Wijesekara I, Kim SK. Biological Activities and Potential Health Benefits of Bioactive Peptides Derived from Marine Organisms. International Journal of Biological Macromolecules. 2012; 51.

Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 Medicinal Plant Extracts for Antioxidant Capacity and Total Phenols. Food Chem. 2006; 94(4).

Ginting B, Mustanir M, Helwati H, Desiyana LS, Eralisa E, Mujahid R. Antioxidant Activity Of n-Hexane Extract of Nutmeg Plants from South Aceh Province. J Nat. 2017;17(1).

Fitra Suloi A, Nur A, Suloi F. Nutmeg (Myristica fragrans Houtt) Bioactivity : Scientific Review Journal of Agricultural Processing Technology. 2021; 3(1): 11-8.

Kim M, Jeon J, Kim J. Streptococcus mutans Extracellular DNA Levels Depend on the Number of Bacteria in a Biofilm. Sci Rep. 2018; 8(1).

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Published

2022-08-31

How to Cite

Dewi Rosmalia, Minarni, & Mhd Riza Marjoni. (2022). Effect of Nutmeg (Myristica Fragrans) Methanolic Extract to the Growth of Dental Plaque Bacteria. DENTA JURNAL KEDOKTERAN GIGI, 16(2), 74–80. https://doi.org/10.30649/denta.v16i2.3

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