Daya Hambat Ekstrak Teripang Pasir (Holonthuria scabra) Terhadap Pertumbuhan Bakteri Mixed periodontopatogen In Vitro

Authors

  • Susi Krestiana Sarjana Kedokteran Gigi Universitas Hang Tuah
  • Widyastuti Periodonsia Fakultas Kedokteran Gigi Universitas Hang Tuah
  • Vivin Ariestania Prostodonsia Fakultas Kedokteran Gigi Universitas Hang Tuah

Keywords:

Holonthuria scabra, periodontal disease, Mixed periodontopatogen

Abstract

Background: Periodontitis is caused by the occupation of gram-negative bacteria (mixed periodontopatogen) in oral cavity. Despite causing resistance, antibiotics are still used as a therapy for periodontitis. Sand sea cucumber (Holonturia scabra) extract hold some
potentials, if developed, to become the alternative therapy for periodontitis due to its antibacterial effects against gramnegative bacteria. Purpose: To determine sand sea cucumber extract (Holonturia scabra) in inhibiting the growth of mixed periodontopatogen bacteria. Materials and Methods: This study used a research design the post-test only control group design. 40 samples of BHI agar plates, contained mixed periodontopatogen bacteria samples, were divided into 5 groups samples. K1 (negative control) was given distilled water containing filter paper, K2 (positive control) was given the antibiotic tetracycline disc 30 g, while P1, P2, and P3 were given a filter paper containing sand sea cucumber extract with a concentration of 150 g/ml, 300 g/ml, and 450 g/ml. All sample from each group were anaerobically inoculated for 2x24 hours. After inoculation, the area around
the filter paper will appear to be clear and it will be measured with digital calipers (mm). The acquired data will be analyzed using the Kruskal-Wallis and Mann-Whitney test. Result: The inhibition rate of K1 (6,00 ± 0,00), K2 (28,975 ± 1,20049), P1 (6,8525 ± 0,30156), P2 (7,6237 ± 0,51281), and P3 (8,75 ± 0,3525). Conclusion: Sand sea cucumber extract (Holonthuria scabra) can inhibit the growth of Mixed periodontopatogen bacteria.

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Published

2021-12-01

How to Cite

Susi Krestiana, Widyastuti, & Vivin Ariestania. (2021). Daya Hambat Ekstrak Teripang Pasir (Holonthuria scabra) Terhadap Pertumbuhan Bakteri Mixed periodontopatogen In Vitro . DENTA JURNAL KEDOKTERAN GIGI, 9(1), 48–58. Retrieved from https://journal-denta.hangtuah.ac.id/index.php/jurnal/article/view/206

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Vol. 9 No. 1 (2015): Februari

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