Socket Preservation with Hydroxyapatite Gipsum Puger Scaffold and Aloe vera on Fibroblast and Type 1 Collagen Cells

Authors

  • Amiyatun Naini Universitas Jember
  • Clarissa Astiasari Faculty of Dentistry, Jember University, Indonesia
  • Muhammad Nurul Amin Department of Biomedicine, Faculty of Dentistry, Jember University, Indonesia

DOI:

https://doi.org/10.30649/denta.v16i1.6

Keywords:

Aloe vera, Gelatin, Gypsum Puger Hydroxyapatite (HAGP), Scaffold, Tooth Extraction

Abstract

Background: The prevalence of tooth extraction was 7.29% according to RISKESDAS 2018. Post-tooth extraction will cause injury and bone resorption. Scaffold material combination of hydroxyapatite gypsum puger (HAGP) and aloe vera (AV) can be used for preservation of extraction socket. Objective: To analyze fibroblast and type 1 collagen cells in the extraction socket after being induction by a combination of hydroxyapatite gypsum puger scaffold and aloe vera. Materials and Methods: Making a combination of gypsum puger hydroxyapatite scaffold and aloe vera, divided into four groups, namely negative control, aloe vera scaffold, HAGP scaffold, and HAGP+AV scaffold. Extraction of the mandibular 1st molar in Wistar rats, the application on the extraction socket was waited for 3, 5, 7, and 14 days. Preparation of preparations using Hemaktocillin Eosin staining method. Results: LSD test on the HAGP+AV scaffold group between days 3 and 5, days 3 and 7, days 3 and 14, days 5 and 7, days 5 and 14, days 7, and 14 were obtained (p=0.000), this shows a significant difference between the treatment groups. The results of type 1 collagen in the HAGP+AV scaffold group between days 3 and 14 were obtained (p=0.005) showing a significant difference. Each group on day 14 showed the highest number of fibroblast and type 1 collagen cells. Conclusion: The combination of HAGP+AV+gelatin scaffold can increase fibroblast cells and type 1 collagen after tooth extraction. The socket healing process is getting faster on day 14.

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Author Biography

Muhammad Nurul Amin, Department of Biomedicine, Faculty of Dentistry, Jember University, Indonesia

Biomedicine Department, Faculty of Dentistry, Jember University, Indonesia

References

Riskesdas K. Hasil Utama Riset Kesehata Dasar (RISKESDAS). J Phys A Math Theor [Internet]. 2018;44(8):1–200. Available from: http://arxiv.org/abs/1011.1669%0Ahttp://dx.doi.org/10.1088/1751-8113/44/8/085201%0Ahttp://stacks.iop.org/1751-8121/44/i=8/a=085201?key=crossref.abc74c979a75846b3de48a5587bf708f

Naini A, Ardhiyanto HB, Yustisia Y. Proses sintesis dan karakterisasi hydoxyapatite menggunakan analisis XRD FTIR dari gypsum puger Kabupaten Jember sebagai material augmentasi ridge alveolar. Stomatognatic (JKG Unej). 2014;11(2):32–7.

Naini A, Sudiana IK, Rubianto M, Kresnoadi U, Latief FDE. Effects of hydroxyapatite gypsum puger scaffold applied to rat alveolar bone sockets on osteoclasts, osteoblasts and the trabecular bone area. Dent J (Majalah Kedokt Gigi). 2019;52(1):13.

Sherman Salim, Mefina Kuntjoro. Efek Kombinasi Spirulina Kitosan Untuk Preservasi Soket Terhadap Osteoblas, Osteoklas Dan Kepadatan Kolagen. Dentika Dent J. 2015;18(3):225–31.

Levina Priscilla. Perbandingan Efektivitas Aplikasi Topikal Lidah Buaya (Aloe vera), Madu (Apis mellifera), dan Kombinasi Keduanya Terhadap Penyembuhan Luka Sayat Pada Mencit (Mus musculus). 2017;17. Available from: http://repository.unhas.ac.id/bitstream/handle/123456789/15556/TINGKAT PENGETAHUAN DAN KESADARAN PELAJAR TERHADAP KANKER RONGGA MULUT.pdf?sequence=1

Novyana RM, Susanti. Lidah Buaya (Aloe vera) untuk Penyembuhan Luka. J Kedokt Univ Lampung. 2016;5:149–53.

Sularsih S, Rahmitasari F. Penggunaan Scaffold Kitosan-Aloe Vera Terhadap Proliferasi Sel Fibroblas pada Penyembuhan Luka Pasca Pencabutan Gigi Cavia Cobaya. J Mater Kedokt Gigi. 2018;7(2):24.

Ramadhani T, Sari RP, W W. Efektivitas Kombinasi Pemberian Minyak Ikan Lemuru (Sardinella longiceps) dan Aplikasi Hidroksiapatit terhadap Ekspresi FGF-2 pada Proses Bone Healing. Denta. 2016;10(1):20.

Kumar S, Kumar Gupta K, Bhowmick D, Singh A. Concepts of Healing in Periodontal Therapy -Part I. IOSR J Dent Med Sci [Internet]. 2015;14(10):2279–861. Available from: www.iosrjournals.org

Turhani D, Weißenböck M, Watzinger E, Yerit K, Cvikl B, Ewers R, et al. In vitro study of adherent mandibular osteoblast-like cells on carrier materials. Int J Oral Maxillofac Surg. 2005;34(5):543–50.

Fitriyana N. Scaffold adalah biomaterial tiga dimensi (3D) yang memfasilitasi sel untuk regenerasi jaringan dan organ. Struktur scaffold harus memiliki porositas tinggi dan saling terhubung sehingga memungkinkan perlekatan sel. Scaffold yang ideal harus memiliki sifat. [Thesis]. 2020;

Hanafiah KA. Rancangan Percobaan Teori dan Aplikasi Edisi Ketiga. Jakarta: Rajawali Post; 2014.

Rahman AO, Humaryanto. Terhadap Level Fibroblast Dan Kolagen Pada Proses Penyembuhan Luka. JAMBI Med J “Jurnal Kedokt dan Kesehatan.” 2019;7:19–25.

Takzaree, NasrinAbbas, Gholamreza Hassanzadeh, Hadjiakhondi, Mohammad Reza Rouini, Azadeh Manayi MMZ. TGF-ß Expression in Aloe Vera Treated Wounds Transforming Growth Factor-ß ( TGF-ß ) Activation in Cutaneous Wounds after Topical Application of Aloe Vera Gel Abstract : Can J Physiol Pharmacol [Internet]. 2016;1–22. Available from: www.nrcresearchpress.com

Dadashzadeh A, Imani R, Moghassemi S, Omidfar K, Abolfathi N. Study of hybrid alginate/gelatin hydrogel-incorporated niosomal Aloe vera capable of sustained release of Aloe vera as potential skin wound dressing. Polym Bull [Internet]. 2020;77(1):387–403. Available from: https://doi.org/10.1007/s00289-019-02753-8

Herda E, Puspitasari D. Tinjauan peran dan sifat material yang digunakan sebagai scaffold dalam rekayasa jaringan. J Mater Kedokt gigi. 2016;1(5):56–63.

Luo Y, Li D, Zhao J, Yang Z, De Kang P. In vivo evaluation of porous lithium-doped hydroxyapatite scaffolds for the treatment of bone defect. Biomed Mater Eng. 2018;29(6):699–721.

Tahmasebi A, Shapouri Moghadam A, Enderami SE, Islami M, Kaabi M, Saburi E, et al. Aloe Vera-Derived Gel-Blended PHBV Nanofibrous Scaffold for Bone Tissue Engineering. ASAIO J. 2020;66(8):966–73.

Zulfah Al-Fa’izah, Yani Corvianindya Rahayu NH. Peran Fibroblas pada Proses Penyembuhan Luka Pasca Pencabutan Gigi (Role of Fibroblast Cells in the Wound Healing Process after Tooth Extraction). 2017;3(3):69–70.

Christine Theoret D, MV, PhD DA. Physiology of Wound Healing. Vol. 99, Praktische Tierarzt. 2018. 792–793 p.

Ashrin MN, Prananingrum W, Yonatasya FD. Pengaruh Bone Graft Senyawa Kalsium Hasil Sintesis Cangkang Kerang Darah (Anadara granosa) dengan Variasi Waktu Sintering terhadap Proliferasi Sel Fibroblas pada Proses Socket Healing. Denta. 2019;13(1):34–43.

Desjardins-Park HE, Foster DS, Longaker MT. Fibroblasts and wound healing: An update. Regen Med. 2018;13(5):491–5.

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Published

2022-02-28

How to Cite

Naini, A., Clarissa Astiasari, & Muhammad Nurul Amin. (2022). Socket Preservation with Hydroxyapatite Gipsum Puger Scaffold and Aloe vera on Fibroblast and Type 1 Collagen Cells . DENTA JURNAL KEDOKTERAN GIGI, 16(1), 32–40. https://doi.org/10.30649/denta.v16i1.6

Issue

Vol. 16 No. 1 (2022): Februari

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