Comparison Between Antibacterial Extract of Gambier (UncariagambirRoxb) and Chlorhexidine 2% to Enterococcus Faecalis
DOI:
https://doi.org/10.30649/denta.v15i2.2Keywords:
Enterococcus faecalis, chlorhexidine 2%, gambier extract (Uncaria gambier Roxb)Abstract
Background: In the case of reinfection after endodontic treatment, Enterococcus faecalis is a bacterium that is commonly found in root canals. Irrigation was carried out to eliminate Enterococcus faecalis from the root canal. Chlorhexidine 2% irrigating agent was effective in reducing the growth of Enterococcus faecalis bacteria and fungi, but it could not dissolve tissue. Gambir extract (UncariagambirRoxb) has antioxidant and antibacterial with phenolic content in the form of catechins. Purpose: Describe the antibacterial comparison of gambier extract (Uncaria gambir Roxb) and 2% chlorhexidine against Enterococcus faecalis bacteria. Material and Methods: This research is a laboratory experimental research. Enterococcus faecalis ATCC 29212 is a microbiological sample. Extract of gambier (UncariagambirRoxb) with concentrations of 1%, 1.5%, 2%, 2.5%, and 3%. 2% chlorhexidine solution and a paper disc to measure the inhibition zone were placed on a petri dish containing Mueller-Hinton agar medium, and incubated 1x24 hours. Gambier extract was made by maceration and rotavapor methods. Results: Gambier extract (UncariagambirRoxb) and 2% chlorhexidine affected the growth inhibition of Enterococcus faecalis bacteria by forming a clear zone around the paper disc in each petri dish. Concentration of 3% is the largest value of the inhibition zone formed in the extract of gambeir (UncariagambirRoxb), while chlorhexidine 2% shows the largest inhibition zone, this can be seen from the average inhibition zone formed of 17.20 mm.Conclussion: There are differences in the antibacterial of gambier extract (UncariagambirRoxb) and 2% chlorhexidine against Enterococcus faecalis bacteria.
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References
Gutmann JL, Paul EL. Problem Solving in Endodontics: Prevention Identification, and Management. 5th Edition. Elsevier/Mosby. 2011. p: 209-16.
Jiang LM, Bram L, Leonardus M, Lucas WMV, Wesselink P. Comparison of the Ceaning of Different Final Irrigation Techniques. J.Endod 2012;38 (6):838-41
Zaparolli D, Saquy PC, Cuz-filho AM. Effect of Sodium Hypochlorite and EDTA Irrigation, Individually and in Alternation, on Dentin Microhardness at the Furcation Area of Mandibular Molars. Brazilian Dental Journal: 2012; 23(6): 654–8.
Cohen, S. & Burns, R.C. Pathway of the pulp (12th ed.). St Louis: Mosby, 2020.
Grossman’s. Endodontic Practice 13th Edition. Wolters Kluwer Health, 2014
Alagl AS, Bedi S, Almas K Phytosolutions for Enterococcus faecalisin Endodontics. Oral Health and Dental Management. 2016;15(5):332-6.
Kumar A, Kumar A, Thakur P,PatilS,Payal C,. Antibacterial activity of green tea(Camellia sinensis) extracts against various bacteria isolated from environmental sources. Recent Research in Science and Technology. 2012;4(1):19-23.
Rahmawati N.,Bakhtiar A., Prima PutraD. Isolasi Katekin dari Gambir (UncariaGambirRoxb) untuk Sediaan Farmasi dan Kosmetik. Jurnal Penelitian Farmasi Indonesia; 2013;1(2).
Azmi Dhalimi, Permasalahan Gambir (UncariagambirRoxb) di Sumatera Barat dan Alternatif Pemecahannya. Perspektif Jurnal Pertanian. 2015. 3; 5(4):46-59.
Hafsah K, Suminarti, Indya KM, Rasmidar S, Moch H, Suryani A. Inhibitory Concentration and Minimun Contact Time Gambir Extract (UncariagambirRoxb) Against Bacterial Growth Enterococcus faecalis. International Journal of Sciences: Basic and Applied Research. 2016;27(3): 239-146.
Merta IW., NuidjaIN,.Mawati NM. Ekstrak gambir memiliki daya hambat terhadap pertumbuhan Staphylococcus aureus secara in vitro. Jurnal Husada. 2013;10: 39-43.
Kresnawati I, Zainuddin A. Aktivitas antioksidan dan antibakteri dari derivate metal ekstrak daun gambir. Jurnal Littri. 2009;15(4):145-151.
Isnawati A, Raini N, Dwi Sampurno O, Mutiatikum D, Widowati L,Gitawati R. Karakteristik Tiga Jenis Ekstrak Gambir (UncariaGambirRoxb). Sumatera Barat. Bul. Peneliti Kesehatan. 2012; 40(4)
Hendri M, Gustri Y, Wilsn H, Yulia H. Pembuatan Konsentrat Polifenol Gambir (UncariaGambirRoxb) Sebagaai Bahan Antioksidan Pangan. Jurnal Riset Industri, 2010:IV(2):71-82
Wijayanti AI. Pengaruh ekstrak gambir (uncariagambirroxb) sebagai alternative bahan larutan irigasi saluran akar terhadap mikroba saluran akar pada gigi nekrosis [Skripsi]. Makassar, Universitas Hasanuddin, 2011.
Suraini, Chairani, Enlita. Uji Aktivitas Anti jamur Ekstrak Gambir (UncariaGambir) terhadap Candida albicans. Scientia jurnal farmasi kesehatan. 2015;5(2)
Chabib L, Triasuti A, irianti RD. Fomulasi Tablet Hisab ekstrak Gambir (UncariagambirRoxb) dengan Variasi Bahan Pengikat Gom Arab. Majalah Obat Tradisional. 2010;15(2):75-79
Ayu Hapsari, M. Uji Aktivitas Antibakteri (+)(–) Katekin dan Gambir (Uncariagambir) terhadap Beberapa Jenis Bakteri Gram Negatif dan Mekanismenya [Skripsi]. Jakarta, Universitas Islam Negeri Syarif Hidayatullah, 2010.
Sukadana, I.M. Senyawa antibakteri golongan flavonoid dari buah belimbing manis(Avverhoa carambola Linn.L). Jurnal Kimia. 2009;(2):109-66.
Mulyawati,Elma.Peran bahan disinfeksi pada perawatan saluran akar. Majalah Kedokteran Gigi. 2011; 18(2):205-9.
Husain N. Efektifitas ekstrak gambir (Uncariagambir) sebagai alternative bahan larutan irigasi saluran akar terhadap pertumbuhan bakteri Entrococcusfaecalis pada konsentrasi dan waktu kontak yang berbeda. [Skripsi] Makassar, Universitas Hasanuddin. 2011
Istiyaningsih Alvia. PengaruhEkstrakGambir (UncariagambirRoxb) sebagai Alternatif Bahan Irigasi Saluran Akar Terhadap Mikroba Saluran Akar pada Gigi Nekrosis. [Thesis] Universitas Hasanuddin. 2012.
Gupta R, Chandavarkar V, Galgali SR, Mishra M. Chlorhexidine, a medicine for all the oral disease. Global Journal of Medicine and Public Health (GJMPH). 2012; 201;1(2):43.
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