Pengaruh Kafein Terhadap Ekspresi RANKL dan Jumlah Osteoklas Pada Pergerakan Gigi Ortodonti

Herniyati H

Abstract


Latar belakang: Pergerakan gigi ortodonti tergantung pada remodeling tulang yang melibatkan osteoklas dan osteoblas. RANKL memiliki peran penting pada osteoklastogenesis dan meningkat selama pergerakan gigi ortodonti. Kafein yang terkandung dalam minuman kopi teh, dan minuman berkarbonasi dapat meningkatkan osteoklastogenesis. Tujuan: untuk menganalisis efek pemberian kafein terhadap ekspresi RANKL dan jumlah osteoklas pada pergerakan gigi ortodonti. Bahan dan Metode: Metode penelitian yang digunakan adalah eksperimental laboratoris, 16 tikus wistar dibagi menjadi 2 kelompok, yaitu kelompok K: tikus diberikan gaya mekanis ortodonti (GMO) dan kelompok P: tikus diberikan GMO dan kafein sebesar 1,37mg / 100g BB dua kali sehari pagi dan sore. GMO pada tikus dilakukan dengan cara pada gigi molar-1( M-1) rahang atas (RA) kanan dan pada kedua gigi insivus RA diberi kawat ligatur dengan diameter 0,20 mm. Kemudian M-1 RA kanan digerakkan ke mesial dengan Niti closed coil spring. Pengamatan dilakukan pada hari ke 15 dengan pemeriksaan histologi untuk menghitung jumlah osteoklas dan pemeriksaan imunohistokimia untuk menentukan ekspresi RANKL. Hasil: Pemberian kafein meningkatkan jumlah osteoklas pada daerah tekanan dan daerah tarikan (p <0,05). Jumlah osteoklas pada daerah tekanan lebih besar daripada daerah tarikan (p <0,05). Pemberian kafein meningkatkan ekspresi  RANKL pada daerah tekanan dan daerah tarikan (p <0,05). Ekspresi RANKL pada daerah tekanan lebih besar daripada daerah tarikan (p <0,05). Simpulan: Pemberian kafein meningkatkan ekspresi RANKL dan jumlah osteoklas pada pergerakan  gigi ortodonti, sehingga dapat menjadi alternatif mempercepat perawatan ortodonti.


Keywords


RANKL, Osteoclastogenesis, Pergerakan gigi ortodonti, kafein.

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References


Brooks PJ, Nilforoushan D, Manolson MF, Simmons CA, and Gong SG. 2009. Molecular markers of early orthodontic tooth movement. Angle Orthodontist, 79(6): 1113–1108.

Nimeri G, Kau CH, Aboe-kheir NS, and Corona R. 2013. Acceleration of tooth movement during orthodonic treatment – a frontier in Orthodontic. Progress in Orthodontic, 42-14.

Kim JH and Kim N. 2014. Regulation of NFATc1 in Osteoclast Differentiation. J Bone Metab, 21(4): 241-233.

Yamaguchi M. 2009. RANK/ RANKL/OPG During Orthodontic Tooth Movement. Orthod Craniofal Res, 12: 119-113.

Teitelbaum SL. 2007. Osteoclasts: what do they do and how do they do it? Am J Patho, 170(2): 427–35.

Wada T, Nakashima T, Hiroshi N, Penninger JM. 2006. RANKL-RANK signaling in osteoclastogenesis and bone disease. Trends Mol Med, 12(1) 17–25.

Zhang F, Wang CL, Koyama Y, Mitsui N, Shionome C, Sanuki R, Suzuki N, Mayahara K, Shimizu N, Maeno M. 2010. Compressive force stimulates the gene expression of IL-17s and their receptors in MC3T3-E1 cells. Connect Tissue Res, 51(5): 359–69.

Garlet TP, Coelho U, Silva JS, Garlet GP. 2007. Cytokine expression pattern in compression and tension sides of the periodontal ligament during orthodontic tooth movement in humans. Eur J Oral Sci, 115(5): 355–62.

Grant M, Wilson J, Rock P. Chapple I. 2013. Induction of cytokines, MMP9, TIMPs, RANKL and OPG during orthodontic tooth movement. Eur J Orthod, 35(5): 644–51.

Shenava S, Nayak KUS., Bhaskar V, Nayak A. 2014. Accelerated Orthodontics-A Review. International Journal of Scientific Study, 1(5): 39-35.

Sukendro S. 2013. Keajaiban Dalam Secangkir Kopi. Yogyakarta: Penerbit Media Pressindo. H. 17.

Peng S, and Chun HY. 2011. Effect of caffeine on alveolar bone remodeling during orthodontic tooth movement in rats. Journal of Tongji University (Medical Science). 03.

Sella R.C, de Mendonça MR, Osmar AC OA, Li AT. 2012. Histomorphic Evaulation of Periodontal Compresion and Tensien sides During Orthodontic Tooth Movement in Rats. J Orthod, 17(3): 108-17.

Krishnan V, Davidovitch Z. 2006. Cellular, molecular, and tissue-level reactions to orthodontic force. American Journal of Orthodontics and Dentofacial Orthopedics, 129(4): 469–e1.

Tzannetou S, Efstratiadis S, Nicolay O, Gribic J, Lamster I. 2008. Comparison of levels of inflammatory mediators IL-1beta and betaG in gingival crevicular fluid from molars, premolars, and incisors during rapid palatal expansion. Am J Orthod Dentofac Orthoped, 133(5): 707-699.

Reis AS, Riberio LGR, Ocarino NM, Goes AlM, and Serakides R. 2015. Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy. BMD Musculoskeletal Disorder, 16: 10.

Hennemann S, Hoff JWVD, and Maltha JC. 2008. Microbiology of Tooth Movement. Eur J Orthod, 30: 306-299.

Leibbrandt, A. and Penninger JM. 2008. RANK/RANKL: regulators of immune responses and bone physiology. Ann. N. Y. Acad. Sci, 1143: 150-123.

Meikle CM. 2006. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. Europian Journal of Orthodontics, 28: 221-40.

Jianru Yi, Zhang L, Yan B, Yang L, Li Y, and Zhao Z. 2012. Drinking coffee may help accelerate orthodontic tooth movement, Department of Dental Implants, State Key Laboratory of Oral Diseases, West China Stomatology Hospital, Sichuan University, Chengdu, Sichuan, China, 3(2): 75-72.

Jianru Y, Boxi Y, Meile L, Yu W, Wei Z, Yu L, and Zhihe Z. 2016. Caffeine may enhance orthodontic tooth movement through increasing osteoclastogenesis induced by periodontal ligament cells under compression. Archives of Oral Biology, 64: 60-51.

Crockett, JC., Mellis, DJ., Scott DI. and Helfrich MH. 2011. New knowledge on critical osteoclast formation and activation pathways from study of rare genetic diseases of osteoclasts: focus on the RANK/RANKL axis. Osteoporos. In., 1: 20-1.

Nakano Y, Yamaguchi M, Fujita S, Asano M, Saito K, and Kasai K. 2011. Expressions of RANKL/RANK and M-CSF/c-fms in root resorption lacunae in rat molar by heavy orthodontic force. Eur J Orthod, 33(4): 335-43.

Zhao Q, Wang Xi, Liu Y, He Aimin, Jia Ruokun. 2010. NFATc1: Functions in osteoclasts, The International Journal of Biochemistry & Cell Biology, 42(5) 579-576.

Xie R, Jagtman AMK, and Maltha JC. 2008. Osteoclast differentiation during experimentally tooth movement by Short-term applications: An immunohistoche-cal study in rats. Acta Odontologica Scandinavica, 66: 320-314.




DOI: http://dx.doi.org/10.30649/denta.v10i1.34

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