Perbedaan Pengaruh Aplikasi Gel Kombinasi Kitosan Berat Molekul Tinggi dan Rendah dengan Ekstrak Lidah Buaya (Aloe vera) Terhadap Kepadatan Kolagen pada Proses Penyembuhan Ulkus Traumatikus

Michelle Suhartono, Sularsih Sularsih, Nafi’ah Nafi’ah

Abstract


Background: Traumatic ulcer is one of the most common oral wounds. Chitosan has mucoadhesive characteristic while Aloe vera containing lignin which is able to penetrate the skin. It is expected that the combined gel of chitosan and Aloe vera will function as wound healing accelerator in traumatic ulcer. Molecular weight is one of the characteristics of chitosan quality. Purpose: the aim of this experiment was to know the density of collagen fibers in wound healing of traumatic ulcer using the combined gel of chitosan with different molecular weight and Aloe vera. Materials and method: 30 Male Rattus Norvegicus were divided into 3 groups. Group I was control group (without chitosan and Aloe vera), group II was given low molecular weight chitosan and Aloe vera, group III was given high molecular weight chitosan and Aloe vera. The groups were given traumatic ulcer making with 4 mm diameter and 2 mm depth. Rats were sacrificed by decapitation on day 3 and 7 then they were examined histopatologically to see the density of collagen fibers. Result: Statistical analysis with Kruskall Wallis and Mann-Whitney U test showed that there were significant difference p<0,05 between high and low molecular weight chitosan with Aloe vera group on day 3 and 7. Conclusion: Chitosan with high molecular weight and Aloe vera were more effective in wound healing of traumatic ulcer because they increase the density of collagen fibers.

 

Keywords:  Combined gel of chitosan and Aloe vera, density of collagen fibers, wound healing.

 

Korespondensi: Sularsih, Bagian Ilmu Biomaterial Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arif Rahman Hakim 150, Telepon 031-5912191.

Keywords


Combined gel of chitosan and Aloe vera, density of collagen fibers, wound healing.

Full Text:

PDF

References


Cunningham SJ and Quinn FB, 2002. Ulcerative Lesions of The Oral Cavity. Available from http://www.utmb.edu/otoref/grnds/Ulcer-oral-021016/Ulcer-oral-021016.pdf.Accessed March 14,2015.

DeLong L, and Burkhart N, 2008. General and Oral Pathology for the Dental Hygienist. Philadephia: Lippincott Williams & Wilkins. p:295-297.

Terry TSI and McDowell J, 2002. Differential Diagnosis: Is it Herpes or Aphthous., Vol. 3 no.1. Available from http://faculty.ksu.edu.sa/Asmaa%20Faden/341%20MDS/Is%20It%20herpes%20OR%20aphthous.pdf. Accessed March 14,2015.

Houston G, 2009. Traumatic Ulcer. Available from http://emedicine.medscape.com/article/1079501-overview. Accessed March 15, 2015.

Marie NM, Nathan BM, Boardman CH, Diegelmann FR, 2008. Biologic Therapeutic and Molecular Profiling to Optimize Wound Healing. Gynecologic Oncology III. p: 87-91.

Novriansyah R, 2008. Perbedaan Kepadatan Kolagen di Sekitar Luka Insisi Tikus Wistar yang Dibalut Kasa Konvensional dan Penutup Oklusif Hidrokoloid Selama 2 dan 14 Hari. Tesis, Universitas Diponegoro, Semarang. Available from http://eprints.undip.ac.id/28847/1/Robin_Novriansyah_Tesis.pdf. Accessed March 16, 2015.

Rahayu NA, 2014. Pengaruh Aplikasi Simvastatin Secara Topikal Terhadap Kepadatan Kolagen Tulang Alveolar Dalam Proses Penyembuhan Periodontitis. Skripsi. Fakultas Kedokteran Gigi Universitas Gajah Mada Yogyakarta. Available from http://etd.repository.ugm.ac.id/index.php?mod=penelitian_detail⊂=PenelitianDetail&act=view&typ=html&buku_id=72191&obyek_id=4.

Budihargono O, Yuliati A, and Rianti D, 2013. Peningkatan Mobilisasi Sel Polimorfonuklear Setelah Pemberian Gel Kitosan 1% pada Luka Pencabutan Gigi Cavia Cobaya. Material Dental Journal Universitas Airlangga 2013 January. p: 1-6.

Kim F, 2004. Physicochemical and Functional Properties of Crawfish Chitosan as Affected by Different Processing Protocols, Thesis, Lousiana State University. p:6-7.

Boddupalli BM, Mohammed ZNK, Nath RA, Banji D, 2010. Mucoadhesive Drug Delivery System : An Overview. J Adv Pharm Technol Res, 1(4):381-387. Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255397/. Accessed April 10, 2015.

Semalty A, 2006. Mucoadhesive Polymers-A Review. Available from http://www.pharmainfo.net/reviews/mucoadhesive-polymers-review.Accessed March 15 2015.

Baitukalova TA, Bogoslovakia OA, Ol’khovskaia IP, Gluschenko NN, Ovsiannikova MN, Lopatin SA, Varlamov VP, 2005. Regenerating Activity and Antibacterial Effect of Low- Molecular –Weight Chitosan. Izv Akad Nauk Ser Biol, 6 : 659-63. Available from http://link.springer.com/article/10.1007/s10525-005-0141-z#page-1. Accessed March 18, 2015.

Ueno H, Nakamura F, Mukarami M, Okumura M, Kadasawa T, Fujinaga T, 2001. Evaluations Effects of Chitosan for the Extracellular Matrix Production by Fibroblast and Growth Factor Production by Macrophages. J. Biomaterials. Vol 22: 2125.

Subramaniam T, Arun S.,Asha Chowdhery, Sreeja Daas, Mishali Gill, 2014. Versatility of Aloe Vera In Dentistry-A review. IOSR Journal of Dental and Medical Sciences. Vol 13, Issue : 10, Ver. III. p:99-100. Available from http://www.iosrjournals.org/iosr-jdms/papers/Vol13-issue10/Version-3/Y01310398102.pdf. Accessed April 6, 2015.

Silva SS, Caridade SG, Mano JF, Reis L, 2013. Effect of crosslinking in Chitosan/Aloe Vera-Based Membranes for Biomedical Applications. Journal Carbohydrate Polymers 98 (2013) 581-588. Available from http://academia.edu/4328497/Effect_of_crosslinking_in_chitosan_aloe_vera-based_membranes_for_biomedical_applications. Accessed March 23,2015.

Aksongur P, Sungur A, Unal S, Iskit AP, Squier CA, Senel S, 2004. Chitosan Delivery Systems for the Treatment of Oral Mucositis: in vitro and in vivo studies. Journal of Controlled Release 98(2004): 269-279. Available from http://www.ncbi.nlm.nih.gov/pubmed/15262418. Accesed 25 August 2015.

Hartisyah OE, 2011. Preparasi dan Karakterisasi Kitosan Suksinat Sebagai Polimer Mukoadhesif Untuk Sediaan Bukal. Skripsi, Universitas Indonesia Depok. p:1.

Prasetyaningrum N, 2010. Pengaruh Pemberian Ekstrak Daun Lidah Buaya (Aloe Vera Linn) Topikal Terhadap Peningkatan IFN-γ, TGF-β dan Fibroblas Pada Proses Percepatan Penyembuhan Luka Pasca Pencabutan Gigi, Thesis, Universitas Airlangga Surabaya. p:2; 9-11; 13.

Sudibyo. 2013. Metodologi Penelitian Aplikasi Penelitian Bidang Kesehatan. Surabaya:Unesa University.

Hidayat AAA, 2011. Metode Penelitian Kesehatan Paradigma Kuantitatif. Surabaya : Health Books Publishing.

Matsunaga S, Yanagiguchi K, Yamada S, Ohara H, 2005. Chitosan Monomer Promotes Tissue Regeneration on Dental Pulp Wounds. J. Biomed. Mater. Res. Vol. 76A. P: 711-720;

Lavoie JP dan Hinchelif K, 2008. Blackwell’s Five-Minute Veterinary Consult: Equine, 2nd ed., Iowa: Wiley-BlackwellP P 162.

Puspita BS, 2015. Perbedaan Pengaruh Pemberian Kitosan BM Tinggi dan Rendah terhadap Jumlah Pembuluh Darah pada Proses Penyembuhan Luka Pencabutan Gigi. Skripsi. Universitas Hang Tuah: Surabaya. p:4, 32

Mahmudah, 2013. Uji Aktivitas Film Kitosan yang Mengandung Asiatioksida sebagai Penutup Luka Bakar pada Tikus Putih Betina (Rattus Novergicus) Galur Sprague Dawley secara In Vivo. Skripsi. Jakarta: Fakultas Kedokteran dan Ilmu Kesehatan Jakarta. p 23.

Greenberg MS, 2008. Ulcerative, Vesicular, and Bulous Lesion in Burket’s Oral Medicine Diagnosis and Treatment. 11th Ed. New York : BC Decker Inc. p:57.

Regezi JA, Sciubba JJ, Jordan RCK, 2008. Oral Pathology Clinical Pathologic Correlations, 5th Edition. St. Louis: Elsevier Science. p:21-24.

DiPietro AL, Guo S, 2010. Factors Affecting Wound Healing. Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2903966/. Accessed April 4, 2015.

Damayanti SA, Budi Y, Dwi MCR, 2012. Efek Pemberian Kurkumin terhadap Pembentukan Kolagen Pada Soket Gigi Tikus Wistar Pasca Pencabutan. Available from http://repository.unej.ac.id/bitstream/handle/123456789/59378/Shelvina%20Ayu%20Damayanti.pdf?sequence=1. Accessed December 15, 2015.

Dai T, et al, 2011. Chitosan Preparations for Wounds and Burns : Antimicrobial and Wound Healing Effects. Expert Rev Anti Infect Ther July 2011 : 9(7): 857-879. Available from. Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188448/. Accessed March 17, 2015.

Budianto B. 2013. Pengaruh Kitosan Gel 1% yang Memiliki Berat Molekul Tinggi dan Rendah Terhadap Jumlah Sel Osteoblas pada Proses Penyembuhan Luka Pnecabutan Gigi. Surabaya: Fakultas Kedokteran Gigi Universitas Hang Tuah. P:57.

Djamaluddin AM, 2009. Pemanfaatan Khitosan dari Limbah Krustasea untuk Penyembuhan Luka pada Mencit (Mus musculus albinus). Skripsi. Institut Pertanian Bogor, Bogor. p:8.

Hritcu D, Popa N, Badescu V, Balan V, 2008. Preparation and Characterization of Magnetic Chitosan Nanospheres. Turk J Chem, 33:787-796. Available from http://journals.tubitak.gov.tr/chem/issues/kim-09-33-6/kim-33-6-7-0812-42.pdf. Accessed April 10, 2015.

Budiman A, 2011. Kelarutan Farmasi Kimia. Available from http://blogs.unpad.ac.id/arifbudiman/files/2011/05/Farmasi-fisika-kelarutan.pdf. Accessed December 21, 2015.

Ratnaningsih R, 2012. Laju Reaksi. Available from http://kimia.upi.edu/staf/nurul/web2012/0905762/isi-materi3.html. Accessed December 21, 2015.

Atik N, Januarsih IAR, 2009. Perbedaan Efek Pemberian Topikal Gel Lidah Buaya (Aloe vera L.) dengan Solusio Povidone Iodine Terhadap Penyembuhan Luka Sayat Pada Kulit Mencit (Mus musculus). Available from http://journal.fk.unpad.ac.id/index.php/mkb/article/download/188/pdf_72. Accessed December 16, 2015.

Triyono, B, 2005. Perbedaan Tampilan Kolagen di Sekitar Luka Insisi pada Tikus Wistar yang Diberi Infiltrasi Penghilang Nyeri Levobupivakain dan yang Tidak Diberi Levobuvipakan (Studi Histokimia). Tesis, Univerisitas Diponegoro, Semarang. Available from http://eprints.undip.ac.id/16709/1/Bambang_Triyono.pdf. Accessed April 4,2015.

Jithendra P, Rajam AM, Kalaivani T, Mandal AB, Rose C, 2013. Preparation and Characterization of Aloe Vera Blended Collagen-Chitosan Composite Scaffold for Tissue Engineering Applications. Available from http://www.ncbi.nlm.nih.gov/pubmed/23838342. Accessed June 18, 2015.

Setiawan K, 2013. Uji Efektifitas Aplikasi Topikal Ekstrak Daun Manggrove Avicennia Marina Terhadap Kepadatan Serabut Kolagen Pada Proses Penyembuhan Ulkus Traumatikus

Ratnawati ID, 2013. Peningkatan Sel Makrofag Pada Luka Pencabutan Gigi Cavia Cobaya Setelah Pemberian Gel Kitosan 1%. Skripsi. Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya. p: 39.

Sari TA, Finsa TS, Anne HD, Arachadian N, Dyah L, 2013. The Effect of Chitosan-Gelatin Membrane on Angiogenesis and Collagen Density in Labial Ulceration Healing. The Indonesian Journal of Dental Research. Available from http://ijdr.fkg.ugm.ac.id/wp-content/uploads/Tita%20Arum%20Sari.pdf. Accessed June 25, 2015.

Sularsih, 2011. Penggunaan Kitosan Dalam Penyembuhan Luka Pencabutan Gigi Rattus Norvegicus. Tesis, Program Magister Kedokteran Gigi, Universitas Airlangga, Surabaya. p: 31-35.

Aranaz I, Mengibar M, Harris R, Panos I, Miralles B, Acosta N, Galed G and Heras A, 2009. Functional Characterization of Chitin and Chitosan. Current Chemical Biology, 2009, 3, 203-230.

Alsarra IA, 2009, Chitosan Topical Gel Formulation in the Management of Burn Wounds. International Journal of Biological Macromolecules 2009; Vol 45:16-21.

Frisca, Sardjono CT, Sandra F, 2009. Angiogenesis: Patofisiologi dan Aplikasi Klinis. JKM, 2009, Vol.8 No.2: 174-187.

Nanci A, 2008. Ten’s Cate Oral Histologi Development Structur and Function. 7th edition. St. Louis Elsevier. p: 63-78; 290-292; 319-357; 380-383.

Velnar, Bailey T, Smrkolj V, 2009. The Wound Healing Process: an Overview of the Cellular and Molecular Mechanisms. The Journal of International Medical Research, 37: 1532-1537. Available from http://imr.sagepub.com/content/37/5/1528.full.pdf. Accessed May 8, 2015.

Hidayat TSN, 2013. Karya Akhir Peran Topikal Ekstrak Gel Aloe vera pada Penyembuhan Luka Bakar Derajat Dalam pada Tikus. Departemen/SMF Ilmu Bedah Plastik Rekonstruksi dan Estetik Fakultas Kedokteran Universitas Airlangga Surabaya.P:38-39.

Femenia A, Pascual PG, Simal S, Rossello C, 2003. Effects of Heat Treatment and Dehydration on Bioactive Polysaccharide Acemannan and Cell Wall Polymers From Aloe Barbadensis Miller. Carbohidrate Polymer 51:397-405. Available from https://www.deepdyve.com/lp/elsevier/effects-of-heat-treatment-and-dehydration-on-bioactive-polysaccharide-wD0rDYP1qA. Accessed April 6, 2015.

Jimmy TNC, David AW, Kenneth MY, Warren JG, 2005. Chemical Characterization of Immunomodulating Polysaccharide of Aloe Vera L. Carbohydrate Research 340:1131-1142.

Tsirogianni AK, Moutsopoulos HM, 2006. Wound Healing : Immunological Aspects. Injury vol 37 Issue 1, April 2006. p: S15-S12. Available from http://www.ncbi.nlm.nih.gov/pubmed/16616753. Accessed March 19,2015.

Davis RH, 2000. The Conductor-Orchestra Concept of Aloe Vera. Available from http://wearewinners.com/wp-content/uploads/2013/10/Conductor-Orchestra-Concept-of-Aloe-Vera.pdf. Accessed March 20,2015.

Subramainan S, Satish KD, Arulselvan P, 2006. Wound Healing Potential of Aloe Vera Leaf Gel Studied in Experimental Rabbits. Asian Journal of Biochemistry 1(2):178-185.

Yuza F, 2013. Efek Pemberian Ekstrak Lidah Buaya (Aloe barbadensis Miller) Pada Soket Gigi Terhadap Kepadatan Serabut Kolagen Pasca Ekstraksi Gigi Marmut (Cavia porcellus). Skripsi. Universitas Gajah Mada Yogyakarta. Available from http://etd.repository.ugm.ac.id/index.php?mod=penelitian_detail⊂=PenelitianDetail&act=view&typ=html&buku_id=62697. Accessed December 16, 2015.

Hardiana D, 2011. Efektivitas Ekstrak Lidah Buaya (Aloe Barbadensis Milleer) Terhadap Proses Penyembuhan Luka Insisi Pada Tikus Wistar. Thesis, Universitas Airlangga Surabaya. p: 3.

Saed, M.A., Ahmad, L., Yaqub, U., Akbar, S., Waheed, A., Saleem, M., Nasirud-Din, 2003. Aloe Vera : A Plant of Vital Significance. Quarterly Science Vision Vol. 9 No. 1-2 Jul-Dec. Available from http://www.sciencevision.org.pk/BackIssues/Vol9/21.aloe_vera.pdf. Accessed April 7, 2015.




DOI: http://dx.doi.org/10.30649/denta.v12i1.156

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Denta Jurnal Kedokteran Gigi

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

 

 

 

View My Stats



 Denta is indexed on:

                                                                          

 

 

                                                                                                    

                                                                        Faculty of Dentistry, Universitas Hang Tuah

                                                    Denta Jurnal Kedokteran Gigi (p-ISSN:1907-5987; e-ISSN:2615-1790)

                           This site is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.